NEUROENDOCRINE MEDIATION OF SOCIALLY INDUCED ANOVULATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 今年的研究仍在继续，评估由社会从属关系引起的心理社会压力对成年雌性恒河猴代谢、生殖和行为结果的影响。此外，还评估了编码5-羟色胺再摄取转运体(5HTT)基因的多态的贡献，因为其他研究表明，携带短启动子长度变异(S变异)的一个或两个等位基因的个体比携带长启动子长度纯合子的雌性个体(L/L)更容易受到心理社会压力的不利影响。在本年度中，评估了雌二醇和孕酮对代谢激素和人体测量的影响。雌二醇显著降低了所有女性的体重和脂肪，无论其社会地位和基因类型如何，这一作用被黄体酮逆转。血清瘦素水平与体重变化平行。 第二项研究评估了一种假说，即给予促肾上腺皮质激素释放激素(CRH)受体类似物会减少促黄体生成素的分泌，特别是在从属动物中。女性服用雌二醇单用或与CRH受体类似物联合使用。下级女性，尤其是S变异的5HTT基因携带者，对雌二醇对黄体生成素的负反馈作用敏感。CRH受体类似物的联合应用增强了所有女性的雌二醇负反馈，甚至在从属女性中的作用更大。 这些研究为患有应激性不孕症的女性提供了可能的治疗策略。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Studies have continued this year assessing the consequences of psychosocial stress, resulting from social subordination, on metabolic, reproductive, and behavioral outcomes I adult female rhesus monkeys. In addition, the contribution of polymorphisms in the gene that encodes the serotonin reuptake transporter (5HTT) was evaluated as other studies show individuals carrying one or both alleles of the short promoter length variant (s-variant) are more vulnerable to the adverse consequences of psychosocial stress than females homozygous for the long promoter length (l/l). During the current year, the effects of estradiol and progesterone on metabolic hormones and anthropometric measures were evaluated. Estradiol significantly reduced body weights and fat in all females, regardless of social status and genotype and this effect was reversed by progesterone. Serum leptin levels paralleled changes in body weight. A second study evaluated the hypothesis that administration of a corticotropin releasing hormone (CRH) receptor analogue would decrease LH secretion, particularly in subordinate animals. Females received estradiol alone or in combination with a CRH receptor analogue. Subordinate females, particularly those with the s-variant 5HTT genotype, were hypersensitive to the negative feedback action of estradiol on LH. Co-administration of the CRH receptor analogue enhanced estradiol negative feedback in all females, and even to a greater degree in subordinate females. These studies provide insights into possible treatment strategies for women suffering from stress-induced infertility.