CAPILLARY HPLC CID MS & INTERPRETATION OF CID SPECTRA

毛细管 HPLC CID MS

• 批准号: 7957358
• 负责人: ALMA L BURLINGAME
• 金额: $ 6.51万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957358
• 关键词: Blood capillaries; Computer Retrieval of Information on Scientific Projects Database; Data; Detection; Funding; Grant; High Pressure Liquid Chromatography; Institution; Mass Spectrum Analysis; Reporting; Research; Research Personnel; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; System; Time; United States National Institutes of Health; capillary; capillary liquid chromatography; instrument; protein aminoacid sequence; research and development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to optimize capillary liquid chromatography using the new Agilent LC and Eksigent system with the ABI/Sciex, ESI QSTAR, ESI-QSTAR XL, and off-line with the MALDI TOF/TOF (4700) instruments so that chromatographic separations followed by mass spectrometric detection and data dependent peptide sequencing can be accomplished routinely at the low femtomole level. (Additional effort and instrument time reported under Collaborative projects and other Technical Research and Development projects.)

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目的目的是使用新型Agilent LC和Eksigent系统以及ABI/Sciex、ESI QSTAR、ESI-QSTAR XL和离线MALDI TOF/TOF（4700）仪器优化毛细管液相色谱，以便在低飞摩尔水平下常规完成色谱分离，然后进行质谱检测和数据依赖性肽测序。 （在合作项目和其他技术研究与开发项目下报告了额外的工作量和仪器时间。）