CAPILLARY HPLC CID MS & INTERPRETATION OF CID SPECTRA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目的目的是优化使用新的安捷伦LC和Eksigent系统与ABI/SCHEX，ESI QSTAR，ESI-QSTAR XL的毛细管液相色谱，并与MALDI TOF/TOF(4700)仪器脱机，以便在低飞摩尔水平上常规地完成层析分离、质谱学检测和数据依赖的多肽测序。 (在协作项目和其他技术研究和开发项目下报告的额外工作量和仪器时间。)

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to optimize capillary liquid chromatography using the new Agilent LC and Eksigent system with the ABI/Sciex, ESI QSTAR, ESI-QSTAR XL, and off-line with the MALDI TOF/TOF (4700) instruments so that chromatographic separations followed by mass spectrometric detection and data dependent peptide sequencing can be accomplished routinely at the low femtomole level. (Additional effort and instrument time reported under Collaborative projects and other Technical Research and Development projects.)