MONOLITHIC CAPILLARY-HPLC & INTRODUCTION OF INTACT PROTEINS & PROTEIN DIGEST T

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目的目的是优化使用整体毛细管液相色谱与QSTAR-XL仪器，使蛋白质的清理和完整的分子量的成功测量，可以在飞摩尔水平常规完成。与其他固定相如C4的性能比较正在进行中。 预计我们将探索制造我们自己的整体柱，并根据我们的研究需求优化性能。 （在合作项目和其他技术研究与开发项目下报告了额外的工作量和仪器时间。）

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to optimize use of monolithic capillary liquid chromatography with the QSTAR-XL instrument so that clean-up of proteins and successful measurements of intact molecular weights can be accomplished routinely at the femtomole level. A comparison of performance with other stationary phases such as C4 is in progress. It is anticipated that we will explore fabrication of our own monolithic columns with properties optimized for our research needs. (Additional effort and instrument time reported under Collaborative projects and other Technical Research and Development projects.)