ENVIRONMENTAL EFFECTS ON SIGNAL TRANSDUCTION

环境对信号传导的影响

• 批准号: 7953841
• 负责人: JEFFREY D LASKIN
• 金额: $ 2.24万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953841
• 关键词: Carcinogens; Cells; Computer Retrieval of Information on Scientific Projects Database; Cytochrome P450; Development; Diffusion; Environment; Enzyme Inhibitor Drugs; Enzyme Inhibitors; Epigallocatechin Gallate; Funding; Grant; Hypoxia; Individual; Institution; Malignant neoplasm of prostate; Molecular Target; Nutrient; Oxidative Stress; Oxygen; Process; Prostate; Prostatic Neoplasms; Proteins; Research; Research Personnel; Resources; Role; Signal Transduction; Source; Tissues; United States National Institutes of Health; Vanillic Acid; biological adaptation to stress; cell type; ferulic acid; interest; monolayer; neoplastic cell; novel; sensor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr. Laskin's current research interests are in understanding the role of dietary nutrients in the development of prostate cancer. In particular, he has been characterizing bioactivation of carcinogens in the prostate, a tissue with limited cytochrome P450 activity. He has identified a novel molecular target for nutrients in the prostate, which is a protein that oxidizes prostate carcinogens to DNA-reactive metabolites and generates cellular oxidative stress. Several important nutrients, in particular, ferulic acid, vanillic acid and epigallocatechin gallate, are effective and potent inhibitors of this enzyme and may suppress the stress response and this may be an important mechanism of their chemoreventative activity. In the BRC, Dr. Laskin has been using a variety of different cell types including prostate tumor cells to characterize the process of oxidative stress. In these studies, which utilized self-referencing oxygen sensors, a major effort has focused on determining if oxygen utilization by individual cells and/or cell monolayers, matches diffusion of oxygen from the surrounding environment. Studies were also initiated to determine if cells are able to recover from hypoxia, a major contributor to the oxidative stress response.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 Laskin博士当前的研究兴趣是了解饮食营养素在前列腺癌发展中的作用。 特别是，他一直在表征前列腺中致癌物的生物活化，这是一种细胞色素P450活性的组织。他已经确定了前列腺营养素的新分子靶标，这是一种将前列腺致癌物氧化为DNA反应代谢物并产生细胞氧化应激的蛋白质。几种重要的营养素，尤其是阿魏酸，香草酸和上瓜酸酯食酸酯，是该酶的有效且有效的抑制剂，可能会抑制应激反应，这可能是其化学预测活性的重要机制。 在BRC中，Laskin博士一直在使用多种不同的细胞类型，包括前列腺肿瘤细胞来表征氧化应激过程。 在利用自我引用的氧气传感器的这些研究中，主要的努力集中在确定单个细胞和/或细胞单层的氧气利用是否匹配氧气从周围环境中的扩散。 还开始了研究以确定细胞是否能够从缺氧中恢复，这是氧化应激反应的主要促成者。