EFFECT OF PROTEASES AND CONTRACTILITY ON CAPILLARY MORPHOGENESIS

蛋白酶和收缩性对毛细血管形态发生的影响

• 批准号: 7956523
• 负责人: Andrew J Putnam
• 金额: $ 0.65万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956523
• 关键词: Blood capillaries; Cells; Coculture Techniques; Computer Retrieval of Information on Scientific Projects Database; Endothelial Cells; Fibrin; Fluorescence; Funding; Gel; Grant; Institution; Laboratories; Morphogenesis; Nature; Peptide Hydrolases; Process; Proteins; Research; Research Personnel; Resources; Role; Signal Transduction; Source; United States National Institutes of Health; angiogenesis; capillary; density; interest; interstitial cell; vasculogenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Endothelial cells and different types of interstitial cells are cocultured within fibrin gels of different densities. The interstitial cells provide proangiogenic signals to the endothelial cells which respond by undergoing a process to form capillaries. This process can occur via two mechanisms, vasculogenesis or angiogenesis depending on the culture conditions. We are interested in the role of proteins which regulate the contractile nature of the cells or matrix degradation and what effects they have on the normal process of capillary morphogenesis.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 内皮细胞和不同类型的间质细胞在不同密度的纤维蛋白凝胶内共培养。 间质细胞向内皮细胞提供促血管生成信号，内皮细胞通过经历形成毛细血管的过程来响应。 该过程可以通过两种机制发生，取决于培养条件的血管发生或血管生成。 我们感兴趣的是调节细胞收缩性质或基质降解的蛋白质的作用，以及它们对毛细血管形态发生的正常过程的影响。