FLIM MEASUREMENTS OF CALCIUM CONCENTRATION IN CELL ORGANELLES

细胞器中钙浓度的薄膜测量

• 批准号: 7956516
• 负责人: Theodora M Mauro
• 金额: $ 0.98万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956516
• 关键词: Calcium; Cells; Computer Retrieval of Information on Scientific Projects Database; Data; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Grant; Human; Institution; Ionomycin; Laboratories; Measurement; Organelles; Research; Research Personnel; Resources; Source; Techniques; United States National Institutes of Health; keratinocyte; response; sensor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human Keratinocytes were transfected with a cameleon calcium sensor and permeabilized with ionomycin. In the presence of calcium, FRET occurs between the cameleon CFP (donor) and YFP (acceptor) unit. In order to quantify the cameleon response to different calcium concentration, FLIM measurements of the donor fluorescence were performed and the data were analized using the phasor plot technique.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 用cameleon钙传感器转染人角质形成细胞并用离子霉素透化。 在钙的存在下，FRET发生在Cameleon CFP（供体）和YFP（受体）单元之间。为了定量分析骆驼对不同钙离子浓度的响应，我们对供体荧光进行了FLIM测量，并使用相量图技术对数据进行了分析。