MECHANISMS OF HEALTH EFFECTS OF EXERCISE IN CHILDREN

运动对儿童健康影响的机制

• 批准号: 7956531
• 负责人: PIETRO R GALASSETTI
• 金额: $ 0.8万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956531
• 关键词: Asthma; Biological Assay; Cell Line; Child; Computer Retrieval of Information on Scientific Projects Database; Diabetes Mellitus; Exercise; Fluorescence; Funding; Glucose; Grant; Growth Factor; HL60; Heat-Shock Proteins 70; Heating; Human; Immune; Institution; Insulin; Laboratories; Lipids; Metabolic syndrome; Molecular; Peroxidases; Physical activity; Proteins; Research; Research Personnel; Resources; Source; Stimulus; United States National Institutes of Health; cytokine; neutrophil

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our laboratory is studying human neutrophils and the HL60 promyelocytic cell line to examine the molecular immune and growth factor mechanisms through which physical activity alters diabetes, asthma, and metabolic syndrome in children. At the LFD, we will be performing immunofluorescent assays to determine colocalization, quantification, and mobilization of various proteins (e.g. HSP70, myeloperoxidase, LFA-1) after exercise-like stimuli (e.g. heat, pH alteration) under mock-dysmetabolic conditions (e.g. high glucose/insulin/lipids/cytokines).

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的实验室正在研究人类中性粒细胞和HL 60早幼粒细胞系，以检查分子免疫和生长因子机制，通过这些机制，体力活动改变儿童的糖尿病，哮喘和代谢综合征。 在LFD，我们将进行免疫荧光测定，以确定在模拟代谢异常条件（例如高葡萄糖/胰岛素/脂质/细胞因子）下运动样刺激（例如热，pH值改变）后各种蛋白质（例如HSP 70，髓过氧化物酶，LFA-1）的共定位，定量和动员。