ANALYSIS OF PARTIALLY RECONSTITUTED KINETOCHORE

部分重建着丝粒的分析

• 批准号: 7957866
• 负责人: Trisha N. Davis
• 金额: $ 0.74万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957866
• 关键词: Animal Model; Biology; Cell Cycle; Cell Death; Cell division; Cells; Chromosome Segregation; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; Congenital Abnormality; Eukaryotic Cell; Funding; Fungal Genome; Genetic Materials; Grant; Human; Institution; Kinetochores; Microtubule-Organizing Center; Microtubules; Modeling; Motor; Plus End of the Microtubule; Process; Protein Dynamics; Proteins; Research; Research Personnel; Resources; Role; Saccharomyces cerevisiae; Source; Structure; United States National Institutes of Health; Yeasts; design; insight; reconstitution; research study; spindle pole body; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Accurate chromosome segregation is required for propagation of all cells. Errors in this process are implicated in oncogenesis, birth defects and cell death. Crucial to proper partitioning of the chromosomes during cell division is the establishment, arrangement and then breakdown of a bipolar spindle. The aim of this grant is to further understand the structure, assembly and dynamics of the proteins that create and control the organization of the spindle in the model organism Saccharomyces cerevisiae. The microtubule organizing center in yeast is the spindle pole body (SPB). The SPB is a dynamic structure that undergoes remodeling in a cell-cycle specific manner. We have identified proteins involved in the remodeling process and will characterize them. We have provided a detailed architectural description of the SPB and will continue our structural analysis. The SPB nucleates and organizes microtubules. We will perform a set of experiments designed to distinguish between two current models for nucleation. Finally, we have found that kinetochores can biorient without being attached to the plus-end of microtubules. New insights into biorientation will be obtained from determining the role of kinetochores, motors and other spindle proteins in the formation of the bipolar spindle when plus-end attachment is not involved. Because many proteins and spindle features are conserved from yeast to human, our studies will inform models of how eukaryotic cells correctly distribute their genetic material.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 所有细胞的繁殖都需要精确的染色体分离。这一过程中的错误与肿瘤发生、出生缺陷和细胞死亡有关。在细胞分裂过程中，染色体正确分配的关键是双极纺锤体的建立、排列和随后的分解。这项资助的目的是进一步了解在模式生物酿酒酵母中创建和控制纺锤体组织的蛋白质的结构，组装和动力学。酵母中的微管组织中心是纺锤体极体（SPB）。SPB是以细胞周期特异性方式经历重塑的动态结构。我们已经确定了参与重塑过程的蛋白质，并将对它们进行表征。我们已经提供了一个详细的建筑描述的SPB，并将继续我们的结构分析。SPB成核并组织微管。我们将进行一组实验，旨在区分两个当前的成核模型。最后，我们发现动粒可以在不附着于微管的正端的情况下双向定向。新的见解bireorientation将获得从确定的作用，着丝粒，电机和其他纺锤体蛋白质在形成的双极纺锤体时，加端附件不参与。由于许多蛋白质和纺锤体特征从酵母到人类都是保守的，我们的研究将为真核细胞如何正确分配其遗传物质的模型提供信息。