MALDI & ESI & LC ESI QQTOF AND LC ESI LTQ-ORBITRAP MS TRAINING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们的实验室广泛使用应用生物系统API QSTAR(Tm)Pulsar I QqTOF质谱仪来表征从合作研究项目中获得的复杂生物样品。在本报告年度，为几名工作人员提供了设计和使用QSTAR的培训，以便通过电喷雾质谱/质谱仪、MALDI-MS/MS和LC-ESI-MS/MS分析样品。他们接受了使用批次参数文件、处理脚本和自动IDA MS/MS功能的培训，这些功能针对典型的蛋白质消化进行了优化。提供了LC说明书，内容包括柱填充方法、LC分离参数和ESI接口的理论和应用。Seward博士准备了一份出色的PowerPoint仪器操作和数据处理指南，当小组成员开始使用这些系统时，该指南将分发给他们。2007年春，Thermo-Fisher LTQ-Orbitrap MS系统安装完成后，Leymarie博士和Infusini博士开始负责该系统，参加了Thermo培训课程，并开始向集团其他成员介绍其硬件和软件特性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our laboratory makes extensive use of the Applied Biosystems API QSTAR(tm) Pulsar i QqTOF mass spectrometers for the characterization of complex biological samples obtained from collaborative research projects. During the current reporting year, several staff members have been provided training on the design and use of the QSTAR for the analysis of samples via ESI-MS/MS, MALDI-MS/MS and LC-ESI-MS/MS. They have been trained in the use of batch parameter files, processing scripts and automated IDA MS/MS functions that have been optimized for typical protein digests. LC instruction has been provided and includes theory and application of column packing methodology, LC separation parameters and ESI interfaces. Dr. Seward prepared an excellent powerpoint guide to instrument operation and data processing that is distributed to all group members when they are beginning to use these systems. After the Thermo-Fisher LTQ-Orbitrap MS system was installed in the spring of 2007, Drs. Leymarie and Infusini took on responsibility for the system, attended the Thermo training course and began to introduce other group members to its hardware and software feaures.