PURIFICATION AND STRUCTURAL ANALYSIS OF GANGLIOSIDES IN MDCK CELLS
PURIFICATION AND STRUCTURAL ANALYSIS OF GANGLIOSIDES IN MDCK CELLS
批准号:
7956057
负责人:
Parastoo Azadi
金额:
$0.25万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-06-01 至 2010-05-31
中文摘要
这个子项目是许多研究子项目中利用
资源由NIH/NCRR资助的中心拨款提供。子项目和
调查员(PI)可能从NIH的另一个来源获得了主要资金,
并因此可以在其他清晰的条目中表示。列出的机构是
该中心不一定是调查人员的机构。
Shiloach博士的团队通过插入唾液酸转移酶基因对MDCK细胞进行了修饰。已知唾液酸基转移酶,
ST6GalnacV将6-连接的Neu5Ac转移到神经节苷脂GM1b,从而将后者转化为GD1a。因此,Dr。
Shiloach希望看到改良的MDCK细胞中神经节苷脂上6-连接唾液酸的增加。
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Dr. Shiloach's group modified MDCK cells by inserting a sialytransferase gene. It is known that sialytransferase,
ST6GalnacV, transfers a 6-linked Neu5Ac to the ganglioside GM1b, thus converting the latter to a GD1a. Therefore, Dr.
Shiloach expects to see an increase of 6-linked sialic acids on gangliosides in the modified MDCK cells.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
A National Glycoscience Resource - CCRC Service and Training
-
批准号:10025496
-
项目类别:
-
资助金额:$74.71万
-
财政年份:2020
-
负责人:Parastoo Azadi
-
依托单位:
A National Glycoscience Resource - CCRC Service and Training
-
批准号:10265506
-
项目类别:
-
资助金额:$74.71万
-
财政年份:2020
-
负责人:Parastoo Azadi
-
依托单位:
A National Glycoscience Resource - CCRC Service and Training
-
批准号:10707084
-
项目类别:
-
资助金额:$74.71万
-
财政年份:2020
-
负责人:Parastoo Azadi
-
依托单位:
Glycan linkage and sequence plus determination of site of glycosylation by permethylation of glycopeptides and MSn analysis in a one pot experiment
-
批准号:9337473
-
项目类别:
-
资助金额:$29.1万
-
财政年份:2016
-
负责人:Parastoo Azadi
-
依托单位:
Glycan linkage and sequence plus determination of site of glycosylation by permethylation of glycopeptides and MSn analysis in a one pot experiment
-
批准号:9166719
-
项目类别:
-
资助金额:$29.1万
-
财政年份:2016
-
负责人:Parastoo Azadi
-
依托单位:
Orbitrap Fusion Tribrid Mass Spectrometer
-
批准号:8734751
-
项目类别:
-
资助金额:$75.0万
-
财政年份:2014
-
负责人:Parastoo Azadi
-
依托单位:
PROTON NMR OF 15 OLIGOSACCHARIDES
-
批准号:8363089
-
项目类别:
-
资助金额:$0.17万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
N-LINKED GLYCOSYLATION SITE MAPPING OF HIV-1 GP120
-
批准号:8363095
-
项目类别:
-
资助金额:$0.61万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
MASS SPECTROMETRY OF GLYCOPROTEINS
-
批准号:8363036
-
项目类别:
-
资助金额:$0.34万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
MONOSACCHARIDE COMPOSITION ANALYSIS BY HPAEC
-
批准号:8363087
-
项目类别:
-
资助金额:$0.17万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
COMPOSITION ANALYSIS BY GC-MS
-
批准号:8361845
-
项目类别:
-
资助金额:$0.18万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
SAX-HPLC OF 6 SAMPLES
-
批准号:8361847
-
项目类别:
-
资助金额:$0.18万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
N-LINKED GLYCOSYLATION SITE MAPPING
-
批准号:8361837
-
项目类别:
-
资助金额:$0.18万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
PROTON NMR OF TWO SAMPLES
-
批准号:8361843
-
项目类别:
-
资助金额:$0.18万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
NMR OF 6 SAMPLES
-
批准号:8361846
-
项目类别:
-
资助金额:$0.18万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
TECHNIQUES FOR CHARACTERIZATION OF CARBOHYDRATE STRUCTURE OF POLYSACCHARIDES
-
批准号:8363034
-
项目类别:
-
资助金额:$0.34万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
LINKED GLYCOSYLS ANALYSIS BY GC-MS
-
批准号:8363065
-
项目类别:
-
资助金额:$0.17万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
N-LINKED GLYCANS PROFILING BY MALDI-TOF-TOF MS
-
批准号:8363100
-
项目类别:
-
资助金额:$0.17万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
N-LINKED OLIGOSACCHARIDE PROFILING BY HPAEC
-
批准号:8363115
-
项目类别:
-
资助金额:$0.17万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
DETERMINATION OF D-MANNITOL, 3 METHYL GLUCOSE AND LACTULOSE
-
批准号:8363099
-
项目类别:
-
资助金额:$0.17万
-
财政年份:2011
-
负责人:Parastoo Azadi
-
依托单位:
海外基金