LASER SCANNING CYTOMETRY FOR T-CELL SUBSETS IN MACAQUES

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。目标：本项目评估激光扫描细胞术（LSC），以分析病毒基因表达（原位杂交测量）和白细胞群的变化（细胞免疫表型测量）在感染猴-人类免疫缺陷病毒（SHIV）的猕猴。因此，这种方法将有助于确定解释新生儿中猿类艾滋病的细胞机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: This project evaluates laser scanning cytometry (LSC) to analyze both viral gene expression (measured by in situ hybridization) and changes in leukocyte populations (measured by cellular immunophenotype) in macaques infected with simian-human immunodeficiency virus (SHIV). Accordingly, this method will help define the cellular mechanism(s) that accounts for simian AIDS in newborns.