Upgrade of 600 MHz NMR spectrometer

申请资金用于升级已有12年历史的600 MHz磁共振光谱仪，配备Bruker Biospin Avance IIINMR控制台、四重共振反向检测QCI CryoProbe和SampleJet自动换样器。所要求的设备将安装在现有的Bruker SpectrSpin主动屏蔽14.1T磁体上。所要求的600 MHz核磁共振光谱仪的升级对于NIH资助的光谱仪主要用户的研究的特定目标以及哥伦比亚大学在生物科学、结构生物学和生物物理学以及临床研究方面的现有优势的长期目标都是至关重要的。在600 MHz仪器上进行的研究范围从生物分子结构/功能的基础研究，例如DNA修复，到分析正常和病理组织中的生物标记物，再到发现新的先导化合物用于药物应用。所需设备的三个组件中的每一个都在性能上提供了重大改进，它们的整体将极大地增强600 MHz核磁共振光谱仪进行这项研究的能力。

DESCRIPTION (provided by applicant): Funding is requested to upgrade a 12-year-old 600 MHz MR spectrometer with a Bruker Biospin AVANCE IIINMR Console, quadruple resonance inverse detection QCI CryoProbe, and SampleJet automated sample changer. The requested equipment will be installed on an existing Bruker Spectrospin actively shielded 14.1 T magnet. The requested upgrades of the 600 MHz NMR spectrometer are essential both to the particular aims of the NIH-funded research of the major users of the spectrometer and to the long-term goal of Columbia University to build upon its existing strengths in biological sciences, structural biology and biophysics, and clinical research. Research conducted on the 600 MHz instrument ranges from fundamental studies of structure/function of biomolecules, for example involved in DNA repair, to analysis of biomarkers in normal and pathological tissues, to discovery of novel lead compounds to pharmaceutical applications. Each of the three components of the requested equipment provides significant improvements in performance that in their entirety will dramatically enhance the capabilities of the 600 MHz NMR spectrometer to conduct this research.