X-Ray Fluorescence Computer Tomography with Emission Tomography Apertures

带发射断层扫描孔径的 X 射线荧光计算机断层扫描

• 批准号: 8142859
• 负责人: Patrick Jean La Riviere
• 金额: $ 18.03万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8142859
• 关键词: Accounting; Algorithms; Animal Model; Animals; Area; Biological; Biological Testing; Biology; Calcium; Calibration; Catalysis; Cells; Characteristics; Collaborations; Collection; Computers; Contrast Media; Coupled; Data; Detection; Development; Diagnosis; Diagnostic; Diagnostic radiologic examination; Discipline; Elements; Fluorescence; Foundations; Generations; Genomics; Goals; Hour; Human; Image; Imaging Techniques; Indium; Individual; Insulin; Investigation; Ions; Islet Cell; Laboratories; Lead; Lighting; Magnetic Resonance Imaging; Manganese; Maps; Metals; Methods; Modeling; Motivation; Pancreas; Photons; Play; Positioning Attribute; Procedures; Reaction; Resolution; Roentgen Rays; Role; Sampling; Scanning; Scheme; Signal Transduction; Slice; Source; Spatial Distribution; Specimen; Speed; Synchrotrons; System; Techniques; Technology; Testing; Therapeutic; Time; Tissue Sample; Trace Elements; Trace metal; Universities; Work; X-Ray Computed Tomography; Zinc; base; beamline; cancer therapy; data acquisition; detector; experience; flexibility; image reconstruction; improved; in vivo; interest; molecular imaging; nanoparticle; novel; optical imaging; public health relevance; reconstruction; scale up; sensor; statistics; tomography; tool

DESCRIPTION (provided by applicant): The overall goal of this proposal is to develop and implement faster and more accurate synchrotron-based X-ray fluorescence computed tomography (XFCT) methods for the mapping of trace metals in biological samples. Many endogenous metals play critical roles in signal transduction and reaction catalysis, while others are quite toxic even in trace quantities. The study of these metals in biology would benefit greatly from the 3D spatially resolved maps of trace element distribution provided by the methods being explored in the proposal. In addition, exogenous metals are often critical components of new in-vivo molecular imaging agents. The techniques proposed here would provide calibration and subcellular localization information critical for the continued advancement of these technologies. XFCT is a stimulated emission tomography (ET) method in which monochromatic synchrotron X-rays are used to stimulate emission of characteristic X-rays from a sample, and it has the ability to produce three-dimensional maps of the distribution of individual elements in a small, intact specimen. As practiced now, the principal limitation of XFCT is its long acquisition time (on the order of 1 or more hours per slice), which limits the ability to image multiple samples for sake of comparison and improved experimental statistics. The key motivation for this proposed effort is to develop a novel detection system for XFCT studies that has a greatly improved imaging speed (10 to 100 times faster) while maintain a reasonable imaging resolution and an excellent sensitivity to the trace elements of interest. The specific aims of the proposal are: 1. To develop an ET-based detection system for XFCT applications. 2. To develop novel XFCT image reconstruction strategies accounting for secondary and scatter-induced fluorescence and that allow for region of interest imaging 3. To test the system and algorithms developed on a problem of biological interest: determining the spatial distribution of manganese introduced in islet cells Upon completion, this project will provide a validated means to perform trace metal imaging in biological specimens at significantly higher throughput than currently achievable. The project will also provide a foundation for scaling the techniques up to in vivo trace metal imaging in animals and humans. PUBLIC HEALTH RELEVANCE: XFCT is a stimulated emission tomography (ET) method in which monochromatic synchrotron X-rays are used to stimulate emission of characteristic X-rays from a sample, and it has the ability to produce three-dimensional maps of the distribution of individual elements in a small, intact specimen. As practiced now, the principal limitation of XFCT is its long acquisition time (on the order of 1 or more hours per slice), which limits the ability to image multiple samples for sake of comparison and improved experimental statistics. The overall goal of this proposal is to develop and implement faster and more accurate synchrotron-based X-ray fluorescence computed tomography (XFCT) methods for the mapping of trace metals in biological samples. Many endogenous metals play critical roles in signal transduction and reaction catalysis, while others are quite toxic even in trace quantities. The study of these metals in biology would benefit greatly from the 3D spatially resolved maps of trace element distribution provided by the methods being explored in the proposal. In addition, exogenous metals are often critical components of new in-vivo molecular imaging agents. The techniques proposed here would provide calibration and subcellular localization information critical for the continued advancement of these technologies.

描述（由申请人提供）：本提案的总体目标是开发和实施更快、更准确的基于同步加速器的 X 射线荧光计算机断层扫描 (XFCT) 方法，用于绘制生物样品中的痕量金属。许多内源金属在信号转导和反应催化中发挥着关键作用，而其他金属即使微量也具有相当的毒性。生物学中对这些金属的研究将大大受益于该提案中探索的方法所提供的微量元素分布的 3D 空间分辨图。此外，外源金属通常是新型体内分子成像剂的关键成分。这里提出的技术将提供对于这些技术的持续进步至关重要的校准和亚细胞定位信息。 XFCT 是一种受激发射断层扫描 (ET) 方法，其中使用单色同步加速器 X 射线来刺激样品发射特征 X 射线，它能够生成小型完整样品中单个元素分布的三维图。目前的实践中，XFCT 的主要限制是其较长的采集时间（每个切片大约 1 小时或更多小时），这限制了为了比较和改进实验统计而对多个样本进行成像的能力。 这项工作的主要动机是开发一种用于 XFCT 研究的新型检测系统，该系统大大提高了成像速度（快 10 至 100 倍），同时保持合理的成像分辨率和对感兴趣的微量元素的出色灵敏度。该提案的具体目标是： 1. 为 XFCT 应用开发基于 ET 的检测系统。 2. 开发新颖的 XFCT 图像重建策略，考虑二次和散射诱导荧光，并允许感兴趣区域成像 3. 测试针对生物学感兴趣的问题开发的系统和算法：确定胰岛细胞中引入的锰的空间分布 完成后，该项目将提供一种经过验证的方法，以比目前可实现的高得多的吞吐量在生物样本中进行痕量金属成像。该项目还将为将该技术扩展到动物和人类体内痕量金属成像奠定基础。 公共健康相关性：XFCT 是一种受激发射断层扫描 (ET) 方法，其中使用单色同步加速器 X 射线来刺激样品发射特征 X 射线，并且能够生成小型完整样品中单个元素分布的三维图。目前的实践中，XFCT 的主要限制是其较长的采集时间（每个切片大约 1 小时或更多小时），这限制了为了比较和改进实验统计而对多个样本进行成像的能力。该提案的总体目标是开发和实施更快、更准确的基于同步加速器的 X 射线荧光计算机断层扫描 (XFCT) 方法，用于绘制生物样品中的痕量金属。许多内源金属在信号转导和反应催化中发挥着关键作用，而其他金属即使微量也具有相当的毒性。生物学中对这些金属的研究将大大受益于该提案中探索的方法所提供的微量元素分布的 3D 空间分辨图。此外，外源金属通常是新型体内分子成像剂的关键成分。这里提出的技术将提供对于这些技术的持续进步至关重要的校准和亚细胞定位信息。