BIOENGINEERING PRIMATE FOLLICLES: FROM IMMATURE EGGS TO LIVE BIRTHS

灵长类卵泡生物工程：从未成熟卵子到活产

• 批准号: 8173220
• 负责人: RICHARD L STOUFFER
• 金额: $ 9.51万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173220
• 关键词: Abdomen; Alginates; Angiogenic Factor; Antral; Architecture; Autologous Transplantation; Biological Preservation; Biomedical Engineering; Biopsy; Cancer Survivor; Computer Retrieval of Information on Scientific Projects Database; Cortex of ovary; Development; Embryo; Embryo Transfer; Exposure to; Female; Fertility; Fertilization; Fertilization in Vitro; Forearm; Funding; Germ Cells; Goals; Graafian Follicles; Grant; Growth; Growth Factor; Health; Hormones; In Vitro; Institution; Live Birth; Macaca; Monitor; Monkeys; Mothers; Oocytes; Ovarian; Ovarian Tissue; Ovary; Pregnancy; Primates; Research; Research Personnel; Resources; Role; Site; Source; Staging; Transplantation; United States National Institutes of Health; Woman; egg; in vivo; nonhuman primate; offspring; oocyte maturation; prevent; reproductive; research study; scaffold

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is part of a Roadmap Consortium (U54 RR02437, T. Woodruff, PI) entitled "The Oncofertility Consortium: Fertility Preservation for Women". This project's goal is to validate options in nonhuman primates for restoring fertility in female cancer survivors by preventing ovarian exposure to the gametotoxic effects of therapy (by removing and preserving biopsies) and returning ovarian tissue, gametes or embryos for fertility. Studies will be performed in monkeys to: (Aim 1) Bioengineer a scaffold that supports the three-dimensional architecture of the follicle and permits coordinated development of the follicle wall and oocyte in vitro; (Aim 2) Evaluate the role of hormones and growth factors in promoting follicle growth and oocyte quality during culture; (Aim 3) Optimize conditions for autotransplantation of ovarian cortex for coordinated follicle growth and oocyte maturation in vivo; and (Aim 4) Assess fertilization and embryonic potential of oocytes derived from in vitro matured follicles and transplanted ovarian cortical follicles in vivo. Immature follicles will be isolated from macaque ovaries and cultured in an alginate matrix. Ovarian cortex will be autotransplanted to accessible sites (abdomen and forearm), and the ability of pro-angiogenic factors to restore ovarian function will be monitored. Mature oocytes produced by follicles in vitro and cortical transplants in vivo will be evaluated for reproductive potential by in vitro fertilization, embryo transfer into surrogate macaque mothers, pregnancy and health of offspring. Initial experiments were successful in growing small follicles for over 30 days in vitro to the antral stage and promoting steroidogenic function.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目是路线图联盟（U54 RR02437，T。Woodruff，PI）的一部分，名为“妇产金协会：妇女的生育能力保存”。该项目的目的是通过防止卵巢暴露于治疗的配子毒性作用（通过去除和保存活检）并返回卵巢组织，配子或胚胎以获得生育力来验证非人类灵长类动物的选择来恢复女性癌症幸存者的生育能力。 研究将在猴子中进行：（ AIM 1）生物工程师的脚手架，支持卵泡的三维结构，并允许在体外进行卵泡壁和卵母细胞的配位发育； （目标2）评估激素和生长因子在培养过程中促进卵泡生长和卵母细胞质量中的作用； （AIM 3）优化卵巢皮层自动移植的条件，用于体内卵泡生长和卵母细胞成熟； （目标4）评估源自体外成熟卵泡和移植的卵巢皮质卵泡的卵母细胞的受精和胚胎潜力。 未成熟的卵泡将从猕猴卵巢中分离出来，并在藻酸盐基质中培养。 卵巢皮层将自动移植到可访问的位点（腹部和前臂），并将监测促血管生成因子恢复卵巢功能的能力。 卵泡在体外和皮质移植体内产生的成熟卵母细胞将通过体外受精，胚胎转移到替代猕猴的母亲，后代的妊娠和健康状况来评估体内的皮质移植。 最初的实验成功地在体外生长30天以上的小卵泡到肛门阶段并促进了类固醇的功能。