ESTABLISHMENT OF SPECIFIC PATHOGEN FREE RHESUS MACAQUE COLONIES

无特定病原体的恒河猴群体的建立

• 批准号: 8173182
• 负责人: Michael K Axthelm
• 金额: $ 39.51万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173182
• 关键词: AIDS Vaccines; Acquired Immunodeficiency Syndrome; Adult; Alleles; Animals; Arts; Assisted Reproductive Technology; Breeding; Cells; Computer Retrieval of Information on Scientific Projects Database; Female; Funding; Future; Genotype; Grant; HIV; Haplotypes; Housing; Immune response; Institution; Macaca mulatta; Major Histocompatibility Complex; Mediating; Modeling; Oregon; Pathogenesis; Primates; Production; Research; Research Personnel; Resources; SIV; Shelter facility; Source; Specific Pathogen Frees; United States National Institutes of Health; Vaccines; Variant; Virus; Virus Diseases; genetic pedigree; germ free condition; immune function; juvenile animal; male; offspring; prevent; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The projected need for Indian rhesus macaques for AIDS-related research exceeds availability from current domestic breeding programs and there is an urgent need to expand breeding programs for future AIDS vaccine and pathogenesis studies. Rhesus macaques with defined major histocompatability complex (MHC) genotypes and known pedigree are becoming increasingly important for research to understand biologic variation in host immune responses and its effects on vaccine strategies and the pathogenesis of AIDS. The objective of this project is to expand the Oregon National Primate Research Center's specific pathogen-free (SPF) Indian rhesus macaque resource and sufficiently characterize their MHC haplotype to permit selected pedigree breeding for MHC class I alleles useful in AIDS research. Specific aims for accomplishing this objective include intensively managing a subpopulation of the Center's SPF Indian rhesus macaque breeding colony to maximize production of genetically diverse females to expand the breeding capacity of the colony. The breeding colony is typed for ten MHC alleles and managed for the production of MHC-defined offspring of known parentage. Both selective breeding of MHC-typed animals and assisted reproduction technology are used to enhance production of future breeder males that are homozygous for the MAMU-A*01 and other alleles important for assessing virus-specific cell-mediated immune function in simian immunodeficiency virus vaccine models for preventing AIDS virus infection. The colony is maintained in state-of-the-art sheltered field cage housing and is comprised of approximately 660 adult and juvenile animals and produces approximately 130 offspring annually.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 预计印度恒河猴对艾滋病相关研究的需求超过了当前国内育种计划的可获得性，因此迫切需要扩大育种计划，以用于未来的艾滋病疫苗和发病机制研究。具有明确的主要组织相容性复合体(MHC)基因型别和已知家系的恒河猴对于研究宿主免疫应答的生物变异及其对疫苗策略和艾滋病发病机制的影响变得越来越重要。该项目的目标是扩大俄勒冈国家灵长类研究中心的无特定病原体(SPF)印度恒河猴资源，并充分鉴定它们的MHC单倍型，以便筛选出对艾滋病研究有用的MHC I类等位基因的家系。实现这一目标的具体目标包括集中管理该中心SPF级印度猕猴繁育群体的一个亚群，以最大限度地生产遗传多样性的雌性猕猴，以扩大该群体的繁育能力。繁殖群体被分型为10个MHC等位基因，并为生产已知亲缘关系的MHC定义的后代而管理。在预防艾滋病病毒感染的猴免疫缺陷病毒疫苗模型中，MHC类型动物的选择性繁殖和辅助生殖技术都被用来提高未来育种者雄性的产量，这些雄性动物是MAMU-A*01和其他等位基因的纯合，这些等位基因对于评估病毒特异性细胞介导的免疫功能非常重要。这个群体被保存在最先进的野外庇护笼舍中，由大约660只成年和幼年动物组成，每年产生大约130只后代。