Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research

扩大 SPF 恒河猴繁殖群以进行艾滋病研究

• 批准号: 9765428
• 负责人: Michael K Axthelm
• 金额: $ 163.28万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9765428
• 关键词: Acquired Immunodeficiency Syndrome; Adult; Animal Model; Animals; Breeding; Communicable Diseases; Development; Disease; Female; Funding; Genetic; Genotype; HIV Infections; Health Sciences; Homologous Gene; Human; Immune response; Immunity; Immunological Models; Immunology; Infectious Diseases Research; Macaca; Macaca mulatta; Major Histocompatibility Complex; Microbial Genetics; Modeling; Opportunistic Infections; Oregon; Pathogenesis; Primates; Production; Request for Applications; Research; Resistance; Resources; SIV; Target Populations; Universities; Vaccine Research; Viral; Viral Vaccines; Virus; Zoonoses; base; design; enzootic; germ free condition; improved; offspring; pre-clinical; simian human immunodeficiency virus; vaccine development; vector vaccine

OVERALL PROJECT SUMMARY “Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research” The Indian rhesus macaque (Macaca mulatta) develops a disease that closely mimics human acquired immunodeficiency syndrome (AIDS) when infected by simian immunodeficiency virus (SIV) or chimeric simian- human immunodeficiency viruses (SHIV), and represents the best animal model for HIV infection. Preclinical vaccine development is heavily dependent on the SIV and SHIV rhesus macaque models. The value and utility of the model are markedly enhanced by improving the level of microbial and genetic characterization. Macaques free of ubiquitous viruses that are homologues of human viruses responsible for opportunistic infections are essential for a growing number of AIDS-related opportunistic infection models and for viral vaccine vector development based on these agents. The utility of macaque models for immunological research has been hindered by the unprecedented complexity of their major histocompatibility complexes. Comprehensive MHC genotyping has the potential to revolutionize the use of macaques in infectious disease research and to guide functional immunology studies. MHC-restricted cellular immune responses are key in protective immunity and resistance to infectious diseases. The comprehensive objective of this application is to increase the capacity of the ONPRC AIDS Research Expanded SPF Breeding Colony to provide genetically characterized Indian-origin rhesus macaques free of a broad number of enzootic and zoonotic agents to enhance the usefulness of the resource for cutting edge opportunistic agent and vaccine research.

整体项目总结 “用于艾滋病研究的扩展SPF恒河猴繁殖群体” 印度恒河猴(Macaca Mulatta)患有一种与人类后天获得性疾病非常相似的疾病 免疫缺陷综合征(AIDS)感染猿猴免疫缺陷病毒(SIV)或嵌合猿猴- 人类免疫缺陷病毒(SIV)，是目前最好的HIV感染动物模型。临床前 疫苗的开发在很大程度上依赖于SIV和SHV恒河猴模型。价值和效用 通过改善微生物和遗传特征的水平，显著提高了模型的可比性。 没有无处不在的病毒的猕猴，这些病毒是导致机会主义的人类病毒的同源物 感染对于越来越多的艾滋病相关机会性感染模型和病毒来说是必不可少的 基于这些制剂的疫苗载体的开发。猕猴模型在免疫学研究中的应用 它们的主要组织相容性复合体前所未有的复杂性阻碍了它们的发展。 全面的MHC基因分型有可能彻底改变猕猴在传染病中的使用 研究并指导功能免疫学研究。MHC限制性的细胞免疫反应是关键 保护性免疫和抵抗传染病。 这项应用的综合目标是增加ONPRC艾滋病研究的能力 扩大SPF繁育群体，为具有遗传特征的印度原生恒河猴提供不受 广泛使用地方病和人畜共患病药物，以提高尖端资源的有用性 机会主义代理人和疫苗研究。