Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research

扩大 SPF 恒河猴繁殖群以进行艾滋病研究

• 批准号: 10338457
• 负责人: Michael K Axthelm
• 金额: $ 171.44万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10338457
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Breeding; Communicable Diseases; Development; Disease; Genotype; HIV Infections; Homologous Gene; Human; Immune response; Immunity; Immunological Models; Immunology; Infectious Diseases Research; Macaca; Macaca mulatta; Major Histocompatibility Complex; Microbial Genetics; Modeling; Opportunistic Infections; Research; Resistance; Resources; SIV; Vaccine Research; Viral Vaccines; Virus; Zoonoses; base; enzootic; improved; pre-clinical; simian human immunodeficiency virus; vaccine development; vector vaccine

Project Summary, Overall “Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research” The Indian rhesus macaque (Macaca mulatta) develops a disease that closely mimics human acquired immunodeficiency syndrome (AIDS) when infected by simian immunodeficiency virus (SIV) or chimeric simian-human immunodeficiency viruses (SHIV), and represents the best animal model for HIV infection. Preclinical vaccine development is heavily dependent on the SIV and SHIV rhesus macaque models. The value and utility of the model are markedly enhanced by improving the level of microbial and genetic characterization. Macaques free of ubiquitous viruses that are homologues of human viruses responsible for opportunistic infections are essential for a growing number of AIDS-related opportunistic infection models and for viral vaccine vector development based on these agents. The utility of macaque models for immunological research has been hindered by the unprecedented complexity of their major histocompatibility complexes. Comprehensive MHC genotyping has the potential to revolutionize the use of macaques in infectious disease research and to guide functional immunology studies. MHC-restricted cellular immune responses are crucial in protective immunity and resistance to infectious diseases. The comprehensive objective of this application is to increase the capacity of the ONPRC AIDS Research Expanded SPF Breeding Colony to provide genetically characterized Indian-origin rhesus macaques free of a broad number of enzootic and zoonotic agents to enhance the usefulness of the resource for cutting edge opportunistic agent and vaccine research.

项目概要，总体 “扩大SPF恒河猴繁殖群用于艾滋病研究” 印度恒河猴（Macaca mulatta）患上了一种与人类非常相似的疾病。 获得性免疫缺陷综合征（艾滋病），感染猿免疫缺陷病毒 (SIV)或嵌合猴-人类免疫缺陷病毒（SHIV）， HIV感染的动物模型。临床前疫苗开发严重依赖SIV 和SHIV恒河猴模型。该模型的价值和实用性显著提高 通过提高微生物和遗传特性的水平。猕猴免费无处不在 作为负责机会性感染的人类病毒的同源物的病毒， 对于越来越多的艾滋病相关的机会性感染模型和病毒感染至关重要。 疫苗载体的发展基于这些代理。猕猴模型的实用性 免疫学研究受到其主要研究领域前所未有的复杂性的阻碍。 组织相容性复合物全面的MHC基因分型有可能 彻底改变猕猴在传染病研究中的使用， 免疫学研究。MHC限制性细胞免疫应答在保护性免疫中至关重要。 免疫力和抗传染病能力。 这一应用的综合目标是提高ONPRC艾滋病的能力 研究扩大SPF育种群，以提供具有遗传特征的印度血统 恒河猴无大量的地方病和人畜共患病原体， 该资源对尖端机会性病原体和疫苗研究的有用性。