Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research

扩大 SPF 恒河猴繁殖群以进行艾滋病研究

• 批准号: 10338457
• 负责人: Michael K Axthelm
• 金额: $ 171.44万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10338457
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Breeding; Communicable Diseases; Development; Disease; Genotype; HIV Infections; Homologous Gene; Human; Immune response; Immunity; Immunological Models; Immunology; Infectious Diseases Research; Macaca; Macaca mulatta; Major Histocompatibility Complex; Microbial Genetics; Modeling; Opportunistic Infections; Research; Resistance; Resources; SIV; Vaccine Research; Viral Vaccines; Virus; Zoonoses; base; enzootic; improved; pre-clinical; simian human immunodeficiency virus; vaccine development; vector vaccine

Project Summary, Overall “Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research” The Indian rhesus macaque (Macaca mulatta) develops a disease that closely mimics human acquired immunodeficiency syndrome (AIDS) when infected by simian immunodeficiency virus (SIV) or chimeric simian-human immunodeficiency viruses (SHIV), and represents the best animal model for HIV infection. Preclinical vaccine development is heavily dependent on the SIV and SHIV rhesus macaque models. The value and utility of the model are markedly enhanced by improving the level of microbial and genetic characterization. Macaques free of ubiquitous viruses that are homologues of human viruses responsible for opportunistic infections are essential for a growing number of AIDS-related opportunistic infection models and for viral vaccine vector development based on these agents. The utility of macaque models for immunological research has been hindered by the unprecedented complexity of their major histocompatibility complexes. Comprehensive MHC genotyping has the potential to revolutionize the use of macaques in infectious disease research and to guide functional immunology studies. MHC-restricted cellular immune responses are crucial in protective immunity and resistance to infectious diseases. The comprehensive objective of this application is to increase the capacity of the ONPRC AIDS Research Expanded SPF Breeding Colony to provide genetically characterized Indian-origin rhesus macaques free of a broad number of enzootic and zoonotic agents to enhance the usefulness of the resource for cutting edge opportunistic agent and vaccine research.

项目概要，总体 “扩大 SPF 恒河猴繁殖群以进行艾滋病研究” 印度恒河猴（Macaca mulatta）患上一种与人类极为相似的疾病 感染猿猴免疫缺陷病毒后患上获得性免疫缺陷综合症（艾滋病） (SIV) 或嵌合猿猴-人类免疫缺陷病毒 (SHIV)，代表了最好的 HIV感染的动物模型。临床前疫苗开发严重依赖SIV 和 SHIV 恒河猴模型。模型的价值和实用性显着增强 通过提高微生物和遗传特征的水平。猕猴无处不在 与引起机会性感染的人类病毒同源的病毒是 对于越来越多的艾滋病相关机会性感染模型和病毒性感染模型至关重要 基于这些试剂的疫苗载体开发。猕猴模型的实用性 免疫学研究因其专业前所未有的复杂性而受到阻碍 组织相容性复合物。综合 MHC 基因分型有潜力 彻底改变猕猴在传染病研究中的使用并指导功能 免疫学研究。 MHC 限制的细胞免疫反应对于保护性至关重要 免疫力和对传染病的抵抗力。 该应用程序的综合目标是提高 ONPRC AIDS 的能力 研究扩大 SPF 育种群体以提供具有遗传特征的印度原产地 恒河猴不含多种地方性和人畜共患病原体，可增强 该资源对于尖端机会性药物和疫苗研究的有用性。