Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research

扩大 SPF 恒河猴繁殖群以进行艾滋病研究

• 批准号: 10338457
• 负责人: Michael K Axthelm
• 金额: $ 171.44万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10338457
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Breeding; Communicable Diseases; Development; Disease; Genotype; HIV Infections; Homologous Gene; Human; Immune response; Immunity; Immunological Models; Immunology; Infectious Diseases Research; Macaca; Macaca mulatta; Major Histocompatibility Complex; Microbial Genetics; Modeling; Opportunistic Infections; Research; Resistance; Resources; SIV; Vaccine Research; Viral Vaccines; Virus; Zoonoses; base; enzootic; improved; pre-clinical; simian human immunodeficiency virus; vaccine development; vector vaccine

Project Summary, Overall “Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research” The Indian rhesus macaque (Macaca mulatta) develops a disease that closely mimics human acquired immunodeficiency syndrome (AIDS) when infected by simian immunodeficiency virus (SIV) or chimeric simian-human immunodeficiency viruses (SHIV), and represents the best animal model for HIV infection. Preclinical vaccine development is heavily dependent on the SIV and SHIV rhesus macaque models. The value and utility of the model are markedly enhanced by improving the level of microbial and genetic characterization. Macaques free of ubiquitous viruses that are homologues of human viruses responsible for opportunistic infections are essential for a growing number of AIDS-related opportunistic infection models and for viral vaccine vector development based on these agents. The utility of macaque models for immunological research has been hindered by the unprecedented complexity of their major histocompatibility complexes. Comprehensive MHC genotyping has the potential to revolutionize the use of macaques in infectious disease research and to guide functional immunology studies. MHC-restricted cellular immune responses are crucial in protective immunity and resistance to infectious diseases. The comprehensive objective of this application is to increase the capacity of the ONPRC AIDS Research Expanded SPF Breeding Colony to provide genetically characterized Indian-origin rhesus macaques free of a broad number of enzootic and zoonotic agents to enhance the usefulness of the resource for cutting edge opportunistic agent and vaccine research.

项目摘要，总体 “扩大的SPF恒河猕猴育种菌落用于艾滋病研究” 印度恒河猕猴（Macaca Mulatta）开发了一种密切模仿人类的疾病 当被猿猴免疫缺陷病毒感染时获得的免疫缺陷综合征（AIDS） （SIV）或嵌合Simian-Human免疫缺陷病毒（SHIV），代表最好的 艾滋病毒感染的动物模型。临床前疫苗的开发在很大程度上取决于SIV 和Shiv Rhesus猕猴模型。该模型的价值和实用性显着增强 通过改善微生物和遗传表征的水平。猕猴无处不在 是负责机会感染的人类病毒的同源物的病毒是 对于越来越多的与艾滋病相关的机会感染模型和病毒的必不可少 疫苗载体基于这些药物的开发。猕猴模型的实用性 免疫研究受到其专业前所未有的复杂性的阻碍 组织相容性复合物。全面的MHC基因分型有可能 彻底改变了猕猴在传染病研究中的使用并指导功能 免疫学研究。 MHC限制的细胞免疫呼吸器对保护至关重要 免疫和抵抗感染性疾病。 该应用的全面目标是提高ONPRC AIDS的能力 研究扩大了SPF育种菌落，以提供一般性化的印度原生物素 恒河猕猴没有大量的enzootic和人畜共患剂来增强 资源对最前沿的机会剂和疫苗研究的有用性。