Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research

项目总结，总体 “用于艾滋病研究的扩展SPF恒河猴繁殖群体” 印度恒河猴(Macaca Mulatta)患上了一种与人类非常相似的疾病 感染猴免疫缺陷病毒时的获得性免疫缺陷综合征(艾滋病) (SIV)或嵌合的猿人免疫缺陷病毒(SHIV)，代表了最好的 HIV感染的动物模型。临床前疫苗的开发严重依赖于SIV 和希夫猕猴模型。该模型的应用价值和实用性明显增强 通过提高微生物和遗传特征的水平。无处不在的猕猴 与导致机会性感染的人类病毒同源的病毒有 对于越来越多的艾滋病相关机会性感染模型和病毒来说是必不可少的 基于这些制剂的疫苗载体的开发。猕猴模型的实用性 免疫学研究因其主要研究领域前所未有的复杂性而受到阻碍 组织相容性复合体。全面的MHC基因分型有可能 革命性地在传染病研究中使用猕猴，并指导功能 免疫学研究。MHC限制性的细胞免疫反应在保护 对传染病的免疫力和抵抗力。 这一应用的综合目标是增加ONPRC艾滋病的能力 研究扩大SPF繁育群体以提供具有印度血统的基因特征 不含多种地方病和人畜共患病物质的恒河猴 该资源对尖端机会主义药物和疫苗研究的有用性。

Project Summary, Overall “Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research” The Indian rhesus macaque (Macaca mulatta) develops a disease that closely mimics human acquired immunodeficiency syndrome (AIDS) when infected by simian immunodeficiency virus (SIV) or chimeric simian-human immunodeficiency viruses (SHIV), and represents the best animal model for HIV infection. Preclinical vaccine development is heavily dependent on the SIV and SHIV rhesus macaque models. The value and utility of the model are markedly enhanced by improving the level of microbial and genetic characterization. Macaques free of ubiquitous viruses that are homologues of human viruses responsible for opportunistic infections are essential for a growing number of AIDS-related opportunistic infection models and for viral vaccine vector development based on these agents. The utility of macaque models for immunological research has been hindered by the unprecedented complexity of their major histocompatibility complexes. Comprehensive MHC genotyping has the potential to revolutionize the use of macaques in infectious disease research and to guide functional immunology studies. MHC-restricted cellular immune responses are crucial in protective immunity and resistance to infectious diseases. The comprehensive objective of this application is to increase the capacity of the ONPRC AIDS Research Expanded SPF Breeding Colony to provide genetically characterized Indian-origin rhesus macaques free of a broad number of enzootic and zoonotic agents to enhance the usefulness of the resource for cutting edge opportunistic agent and vaccine research.