Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research

扩大 SPF 恒河猴繁殖群以进行艾滋病研究

• 批准号: 9536982
• 负责人: Michael K Axthelm
• 金额: $ 37.18万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9536982
• 关键词: Acquired Immunodeficiency Syndrome; Adult; Animal Model; Animals; Breeding; Communicable Diseases; Development; Disease; Female; Funding; Genetic; Genotype; HIV Infections; Health Sciences; Homologous Gene; Human; Immune response; Immunity; Immunological Models; Immunology; Infectious Diseases Research; Macaca; Macaca mulatta; Major Histocompatibility Complex; Microbial Genetics; Modeling; Opportunistic Infections; Oregon; Pathogenesis; Primates; Production; Request for Applications; Research; Resistance; Resources; SIV; Target Populations; Universities; Vaccine Research; Viral; Viral Vaccines; Virus; Zoonoses; base; design; enzootic; germ free condition; improved; offspring; pre-clinical; simian human immunodeficiency virus; vaccine development; vector vaccine

OVERALL PROJECT SUMMARY “Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research” The Indian rhesus macaque (Macaca mulatta) develops a disease that closely mimics human acquired immunodeficiency syndrome (AIDS) when infected by simian immunodeficiency virus (SIV) or chimeric simian- human immunodeficiency viruses (SHIV), and represents the best animal model for HIV infection. Preclinical vaccine development is heavily dependent on the SIV and SHIV rhesus macaque models. The value and utility of the model are markedly enhanced by improving the level of microbial and genetic characterization. Macaques free of ubiquitous viruses that are homologues of human viruses responsible for opportunistic infections are essential for a growing number of AIDS-related opportunistic infection models and for viral vaccine vector development based on these agents. The utility of macaque models for immunological research has been hindered by the unprecedented complexity of their major histocompatibility complexes. Comprehensive MHC genotyping has the potential to revolutionize the use of macaques in infectious disease research and to guide functional immunology studies. MHC-restricted cellular immune responses are key in protective immunity and resistance to infectious diseases. The comprehensive objective of this application is to increase the capacity of the ONPRC AIDS Research Expanded SPF Breeding Colony to provide genetically characterized Indian-origin rhesus macaques free of a broad number of enzootic and zoonotic agents to enhance the usefulness of the resource for cutting edge opportunistic agent and vaccine research.

总体项目摘要 “扩大SPF恒河猴繁殖群用于艾滋病研究” 印度恒河猴（Macaca mulatta）患上了一种疾病， 免疫缺陷综合征（艾滋病），当感染猿猴免疫缺陷病毒（SIV）或嵌合猿猴- 人免疫缺陷病毒（SHIV），并且代表HIV感染的最佳动物模型。临床前 疫苗开发严重依赖于SIV和SHIV恒河猴模型。价值和效用 通过提高微生物和遗传特性的水平，该模型的性能得到显著增强。 猕猴没有普遍存在的病毒，这些病毒是负责机会性感染的人类病毒的同源物 感染对于越来越多的艾滋病相关的机会性感染模型和病毒感染是必不可少的。 疫苗载体的发展基于这些代理。猕猴模型在免疫学研究中的应用 由于其主要组织相容性复合体的空前复杂性而受到阻碍。 全面的MHC基因分型有可能彻底改变猕猴在传染病中的使用 研究和指导功能免疫学研究。MHC限制性细胞免疫反应是 保护性免疫力和对传染病的抵抗力。 这项申请的综合目标是提高ONPRC艾滋病研究的能力 扩大SPF繁殖群，以提供遗传特征明确的印度恒河猴， 广泛的地方病和人畜共患病的代理人，以提高资源的用途， 机会因子和疫苗研究。