FUNCTIONAL ORGANIZATION OF AUDITORY CORTEX

听觉皮层的功能组织

• 批准号: 8172420
• 负责人: TODD M PRUESS
• 金额: $ 5.48万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172420
• 关键词: Agglutinins; Animals; Antibodies; Area; Auditory area; Cells; Computer Retrieval of Information on Scientific Projects Database; Evolution; Funding; Glutamate Transporter; Grant; Histological Techniques; Histology; Human; Institution; Lateral Geniculate Body; Macaca; Medial; Monkeys; Pan Genus; Parvalbumins; Pattern; Reporting; Research; Research Personnel; Resources; Scanning; Slide; Source; Staining method; Stains; Techniques; Temporal Lobe; Tissue Banks; United States National Institutes of Health; Visual; Wisteria; comparative; neuroimaging

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project seeks to understand the organization of auditory cortex in humans and the evolution of human-specific features of auditory cortex by comparing humans with chimpanzees (the animals most closely related to humans) and in macaque monkeys. We used neuroimaging and histological techniques to define higher-order auditory areas in humans, identify homologous areas in chimpanzees and macaques, and determine patterns of connectivity between auditory areas in the three species. During the reporting period, we built a comparative tissue collection to document the histology of primary and secondary auditory areas in macaques and chimpanzees. This involved sectioning two temporal lobes for each species, staining sets of sections for each species for Nissl, parvalbumin, and Wisteria florabunda agglutinin (WFA), and then scanning whole sections using the Center's Aperio slide scanning facility. This tissue collection is accessible for remote study by our colleagues at Vanderbilt and Nathan Kline. In addition, we stained sections of visual and auditory cortex with antibodies for glutamate transporters (VGLUT1, VGLUT2), These revealed the distribution of terminals originating in cells from the lateral geniculate and medial geniculate. This makes it possible to study the organization of thalamocortical connections in species that cannot be studied with invasive tracing techniques, specifically chimpanzees and humans.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目通过比较人类与黑猩猩（与人类关系最密切的动物）和猕猴，来了解人类听觉皮层的组织结构和人类特有的听觉皮层特征的演变。 我们使用神经成像和组织学技术来定义人类的高阶听觉区域，识别黑猩猩和猕猴的同源区域，并确定这三个物种听觉区域之间的连接模式。 在本报告所述期间，我们建立了一个比较组织收集，以记录猕猴和黑猩猩初级和次级听觉区的组织学。 这涉及到每个物种的两个颞叶切片，对每个物种的切片进行Nissl，小清蛋白和紫藤凝集素（WFA）染色，然后使用该中心的Aperio载玻片扫描设备扫描整个切片。 我们的同事在范德比尔特和内森·克莱恩可以对这些组织进行远程研究。 此外，我们用谷氨酸转运体（VGLUT 1，VGLUT 2）的抗体对视觉和听觉皮层切片进行染色，这些染色揭示了起源于外侧膝状体和内侧膝状体细胞的终末的分布。 这使得研究不能用侵入性追踪技术研究的物种的丘脑皮质连接的组织成为可能，特别是黑猩猩和人类。