PROGESTERONE RECEPTORS & ACTION IN PRIMATE OVARY

黄体酮受体

• 批准号: 8173179
• 负责人: RICHARD L STOUFFER
• 金额: $ 7.61万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173179
• 关键词: Binding Proteins; Cell physiology; Computer Retrieval of Information on Scientific Projects Database; Corticotropin-Releasing Hormone; Development; Enzymes; Fertility; Funding; Gene Proteins; Genes; Graafian Follicles; Grant; Hormonal; Institution; Longevity; Luteinizing Hormone; Luteolysis; Menstrual cycle; Molecular; Monkeys; Ovarian; Ovary; Ovulation; Peptide Hydrolases; Periodicity; Pituitary Hormones; Primates; Process; Progesterone; Progesterone Receptors; Proteins; Research; Research Personnel; Resources; Source; Steroids; Structure; System; Testing; Tissues; United States National Institutes of Health; Woman; corpus luteum; genome-wide; protein expression; receptor binding; receptor expression; research study; steroid hormone; urocortin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this research is to understand the mechanisms whereby hormonal and local factors control the structure-function of the ovulatory follicle and corpus luteum in the ovary, and hence fertility, during the menstrual cycle in primates. Ongoing research is testing the hypothesis that the vital actions of the pituitary hormone, luteinizing hormone (LH) in promoting ovulation of the mature follicle and its subsequent conversion into the corpus luteum, and in controlling the functional lifespan of the corpus luteum, is either direct or indirect via local steroid action (e.g., via stimulation of progesterone [P] synthesis and P receptor expression). Because of similarities to ovarian function in women, monkeys are used in experiments where either LH and steroid hormones are depleted or steroids alone are depleted and replaced. The ovulatory follicle or corpus luteum is then removed to analyze molecular and cellular processes that are LH-dependent and either steroid/P-independent or -dependent. Gene and protein expression are analyzed for various processes, including: (1) protease enzymes that may be critical for tissue remodeling during ovulation, luteal development and luteal regression, and (2) local regulatory systems, e.g., the corticotropin-releasing hormone (CRH)/urocortins (UCN)-receptor-binding protein system. Current genome-wide analyses are identifying processes that are regulated by LH and/or steroid hormones in the follicle of corpus luteum. The activity of these processes is being manipulated, via gene knockdown or protein antagonist approaches, to elucidate their biologic relevance to primate ovarian function and cyclicity.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 这项研究的目的是了解荷尔蒙和局部因素控制卵巢中排卵卵泡和叶黄素的结构功能的机制，从而在灵长类动物的月经周期期间生育力。正在进行的研究正在检验以下假说：垂体激素的重要作用，黄体生成激素（LH）在促进成熟卵泡的排卵及其随后的转化为叶酸肠胃的排卵方面，并通过局部静脉表达（E.G.G.G.G.的表达）来控制luteum的功能寿命，以控制lute的寿命。由于与女性卵巢功能相似，因此在LH和类固醇激素耗尽或单独使用类固醇的实验中使用了猴子。然后，除去排卵毛囊或黄体，以分析LH依赖性且类固醇/P独立或依赖性的分子和细胞过程。分析了基因和蛋白质表达的各种过程，包括：（1）蛋白酶在排卵，黄体发育和黄体回归过程中可能至关重要的蛋白酶，以及（2）局部调节系统，例如皮质激素释放的激素（CRH）/UROCOROCORTIS（UCETTINS） - 蛋白质系统 - 蛋白质蛋白酶 - 蛋白质 - 蛋白质 - 蛋白质 - 蛋白质 - 蛋白质。当前的全基因组分析正在识别由LH和/或类固醇激素调节的过程中的过程。这些过程的活性是通过基因敲低或蛋白质拮抗剂方法来操纵的，以阐明它们与灵长类动物卵巢功能和循环性的生物学相关性。