A Laser Microbeam Biotechnology Resource

描述(申请人提供)：激光微束和医学计划(LAMMP)是美国国立卫生研究院生物医学技术资源中心，致力于激光和光学在生物学和医学中的使用。LAMP位于贝克曼激光研究所(BLI)内，这是加州大学欧文分校的一个跨学科生物医学研究、教学和临床设施。总体资源目标是促进一个平衡的中心，在技术研究和开发、合作研究、服务、培训和传播方面开展活动。在LAMP的第六次续签申请中，我们继续强调我们独特的能力，通过快速将基础科学和技术发现从黑板到台面再到床边来促进“转化性”研究。这是通过将最先进的光学技术与细胞和组织工程、组织病理学、临床前动物模型和临床护理的专门资源设施相结合来实现的。LAMP提供显微镜和微束技术(MMT)，用于活细胞和组织的高分辨率功能成像和操作，以及医学转换技术(MTT)，用于非和微创监测、治疗和成像临床前动物模型和人类受试者。此外，我们建议建立一个新的核心，虚拟光子学技术(VPT)，用于开发计算模型和方法，以提高生物光子学技术的性能，并增强来自光学测量的信息含量。提出了7个技术研究和开发项目，这些项目将导致几个新仪器的制造以及多功能软件的创建。这些项目建立在我们在光-组织相互作用模型和生物光子学技术方面的长期专业知识基础上，包括激光微束、非线性显微镜、光学相干断层扫描、空间/时间调制方法和漫反射光学。LAMMP芯包含能够定量表征、成像和干扰细胞和组织中的结构和生化功能的补充技术，具有可扩展的分辨率和从微米到厘米的深度灵敏度。提出了合作研究，以促进这些技术的发展，使它们成为广泛可用的，使生物学和医学中的方法成为可能。在整个赠款过程中，我们强调LAMMP技术在癌症、心血管疾病、代谢综合征和神经功能以及基本生物过程(如机械转导、伤口修复、血管生成、纤维化和细胞死亡)等领域与医学的相关性。

DESCRIPTION (provided by applicant): The Laser Microbeam and Medical Program (LAMMP) is a NIH Biomedical Technology Resource Center dedicated to the use of lasers and optics in biology and medicine. LAMMP is located within the Beckman Laser Institute (BLI), an interdisciplinary biomedical research, teaching, and clinical facility at the University of California, Irvine. Overall resource objectives are to promote a well-balanced Center with activities in technological research and development, collaborative research, service, training, and dissemination. In this sixth renewal application of LAMMP, we continue to emphasize our unique capabilities to facilitate "translational" research by rapidly moving basic science and technology discoveries from "blackboard to benchtop to bedside". This is accomplished by combining state of the art optical technologies with specialized resource facilities for cell and tissue engineering, histopathology, pre-clinical animal models, and clinical care. LAMMP provides both Microscopy and Microbeam Technologies (MMT) for high-resolution functional imaging and manipulation of living cells and tissues and Medical Translational Technologies (MTT) for non- and minimally-invasive monitoring, treating, and imaging pre-clinical animal models and human subjects. In addition, we propose to establish a new core, Virtual Photonics Technologies (VPT) for developing computational models and methods that advance the performance of biophotonic technologies, and enhance the information content derived from optical measurements. Seven Technology Research and Development projects are proposed that will result in the fabrication of several new instruments as well as the creation of multi-functional software. These projects build on our longstanding expertise in light-tissue interaction models and Biophotonics technologies, including laser microbeams, non-linear microscopy, optical coherence tomography, spatial/temporal modulation methods, and diffuse optics. LAMMP cores contain complementary technologies that are capable of quantitatively characterizing, imaging, and perturbing structure and biochemical function in cells and tissues with scalable resolution and depth sensitivity ranging from micrometers to centimeters. Collaborative studies are proposed that advance these technologies so they become widely-available, enabling methods in Biology and Medicine. Throughout the grant we emphasize the relevance of LAMMP technologies to Medicine in areas such as cancer, cardiovascular disease, metabolic syndrome, and neurologic function, as well as fundamental biological process, such as mechano-transduction, wound repair, angiogenesis, fibrosis, and cell death.