IBR TRANSGENIC ANIMAL CORE

IBR 转基因动物核心

• 批准号: 8360319
• 负责人: WILLIAM S WARD
• 金额: $ 33.59万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360319
• 关键词: Biogenesis; Centers of Research Excellence; Core Facility; Faculty; Funding; Gene Transfer Techniques; Genes; Grant; Hawaii; Institutes; Intracytoplasmic Sperm Injections; Knockout Mice; Modification; Mus; National Center for Research Resources; Principal Investigator; Production; Research; Research Infrastructure; Research Personnel; Resources; Scientist; Source; Techniques; Technology; Transgenic Mice; Transgenic Organisms; Transposase; United States National Institutes of Health; Universities; base; cost; embryonic stem cell; member; ward

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Transgenic Mouse Core will draw upon the expertise of Institute for Biogenesis Research (IBR) faculty members to develop a core facility that augments institutional capacity at the University of Hawai`i at Manoa (UH Manoa). We will create a state-of-the-art Transgenic Mouse Core (TGMC) through using technology developed at the IBR. Four of the five junior investigators, Drs. Alarcon, Moisyadi, M. Ward, and Yamazaki, have mastered the techniques required for mouse transgenesis that were developed at the IBR by Dr. Ryuzo Yanagimachi. More recently, junior investigator Dr. Stefan Moisyadi has developed several modifications of ICSI-Tr that employ transposases to increase the efficiency of transgenic techniques. This will be the focus of Dr. Moisyadi's Project 5. Finally, junior investigator, Dr. Yamazaki, is very proficient in growing and manipulating mouse embryonic stem cells, which are critical to the production of so-called "knock-out mice" in which a gene is removed from the mouse rather than added. The rationale for developing the Transgenic Mouse Core is based on the current needs of both the junior investigators and other researchers at UH-M
noa and on the unique expertise that already exists in the IBR. A Transgenic Mouse Core would be a unique mechanism to enhance the institutional capacity of the entire University of Hawaii while providing an important resource to the junior investigators of the IBR COBRE. Moreover, the IBR contains the only group of scientists within UHM
noa and indeed in the State of Hawai`i, who have the expertise to develop such a core.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 转基因小鼠核心将利用生物发生研究所（IBR）教职员工的专业知识，开发一个核心设施，增强夏威夷大学马诺阿分校（UH Manoa）的机构能力。 我们将通过使用IBR开发的技术创建最先进的转基因小鼠核心（TGMC）。 五名初级研究人员中的四名，阿拉尔孔博士，莫伊西亚迪，M。沃德和山崎已经掌握了由柳町龙三博士在IBR开发的小鼠转基因技术。 最近，初级研究员Stefan Moisyadi博士开发了几种ICSI-Tr的修饰，这些修饰采用转座酶来提高转基因技术的效率。 这将是Moisyadi博士项目5的重点。 最后，初级研究员山崎博士非常精通培养和操纵小鼠胚胎干细胞，这对生产所谓的“敲除小鼠”至关重要，在这种小鼠中，基因被从小鼠中移除而不是添加。 开发转基因小鼠核心的基本原理是基于UH-M的初级研究人员和其他研究人员的当前需求
noa和IBR已经存在的独特专业知识。 转基因小鼠核心将是一个独特的机制，以提高整个夏威夷大学的机构能力，同时提供了一个重要的资源，IBR眼镜蛇的初级研究人员。 此外，IBR包含UHM内部唯一的科学家小组，
诺阿，事实上在夏威夷州，谁拥有开发这样一个核心的专业知识。