IBR TRANSGENIC ANIMAL CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 转基因小鼠核心将利用生物发生研究所（IBR）教职员工的专业知识，开发一个核心设施，增强夏威夷大学马诺阿分校（UH Manoa）的机构能力。 我们将通过使用IBR开发的技术创建最先进的转基因小鼠核心（TGMC）。 五名初级研究人员中的四名，阿拉尔孔博士，莫伊西亚迪，M。沃德和山崎已经掌握了由柳町龙三博士在IBR开发的小鼠转基因技术。 最近，初级研究员Stefan Moisyadi博士开发了几种ICSI-Tr的修饰，这些修饰采用转座酶来提高转基因技术的效率。 这将是Moisyadi博士项目5的重点。 最后，初级研究员山崎博士非常精通培养和操纵小鼠胚胎干细胞，这对生产所谓的“敲除小鼠”至关重要，在这种小鼠中，基因被从小鼠中移除而不是添加。 开发转基因小鼠核心的基本原理是基于UH-M的初级研究人员和其他研究人员的当前需求
noa和IBR已经存在的独特专业知识。 转基因小鼠核心将是一个独特的机制，以提高整个夏威夷大学的机构能力，同时提供了一个重要的资源，IBR眼镜蛇的初级研究人员。 此外，IBR包含UHM内部唯一的科学家小组，
noa和夏威夷州，谁拥有开发这样一个核心的专业知识。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Transgenic Mouse Core will draw upon the expertise of Institute for Biogenesis Research (IBR) faculty members to develop a core facility that augments institutional capacity at the University of Hawai`i at Manoa (UH Manoa). We will create a state-of-the-art Transgenic Mouse Core (TGMC) through using technology developed at the IBR. Four of the five junior investigators, Drs. Alarcon, Moisyadi, M. Ward, and Yamazaki, have mastered the techniques required for mouse transgenesis that were developed at the IBR by Dr. Ryuzo Yanagimachi. More recently, junior investigator Dr. Stefan Moisyadi has developed several modifications of ICSI-Tr that employ transposases to increase the efficiency of transgenic techniques. This will be the focus of Dr. Moisyadi's Project 5. Finally, junior investigator, Dr. Yamazaki, is very proficient in growing and manipulating mouse embryonic stem cells, which are critical to the production of so-called "knock-out mice" in which a gene is removed from the mouse rather than added. The rationale for developing the Transgenic Mouse Core is based on the current needs of both the junior investigators and other researchers at UH-M
noa and on the unique expertise that already exists in the IBR. A Transgenic Mouse Core would be a unique mechanism to enhance the institutional capacity of the entire University of Hawaii while providing an important resource to the junior investigators of the IBR COBRE. Moreover, the IBR contains the only group of scientists within UHM
noa and indeed in the State of Hawai`i, who have the expertise to develop such a core.