Nanoscale energy production for implantable medical devices

用于植入式医疗设备的纳米级能量生产

• 批准号: 8118429
• 负责人: ALEXANDER J TRAVIS
• 金额: $ 76.23万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8118429
• 关键词: Address; Binding; Biological; Coupled; Data; Devices; Enzymes; Germ; Glucose; Glycolysis; Hybrids; Mechanics; Medical; Medical Device; Modeling; Natural regeneration; Pathway interactions; Pharmaceutical Preparations; Physiology; Production; Recombinants; Rest; Series; System; Technology; Testing; Tissues; Work; abstracting; design; enzyme activity; implantable device; innovation; nanobiotechnology; nanodevice; nanoscale; protein function; public health relevance; sperm cell

DESCRIPTION Abstract Nanobiotechnology offers the possibility of new forms of medical treatments, such as implantable devices that carry out biological or mechanical functions, or deliver drugs to specific tissues. Because proteins function so efficiently at this small scale, they will likely be major components of nanodevices. However, a number of important obstacles must be overcome for nanodevices to realize their potential. One of the most critical problems is how to supply implantable nanodevices with energy. Our work on the physiology of mammalian sperm has inspired us with a strategy to address this important issue. Sperm generate ATP throughout the flagellar principal piece by using glycolytic enzymes tethered to a cytoskeletal support by means of germ cellspecific targeting domains. We hypothesize that by identifying and modifying these domains, we can generate recombinant glycolytic enzymes that can be bound to a support and retain function. As proof of principle, we have made modified forms of the first two enzymes in this pathway, and show their activities in series when coupled to the same support. To our knowledge, this is the first demonstration of sequential enzymatic activities in a multi-step pathway on a hybrid organic-inorganic device. These data also support our hypothesis that sperm provide a natural model of how to produce ATP locally on nanodevices. We propose to construct similarly modified recombinant forms of the rest of the enzymes of glycolysis, as well as an additional enzyme that will be needed for co-enzyme regeneration. We shall then test the activities of these enzymes individually, in sub-assemblies, and in series on single supports in our effort to design a system through which implantable nanodevices can produce their own energy from freely available circulating glucose. If successful, our innovative strategy will produce an enabling technology that should advance a variety of medical applications for nanobiotechnology. Public Health Relevance

描述

项目成果

Activation of actin-cardiac myosin subfragment 1 MgATPase rate by Ca2+ shows cooperativity intrinsic to the thin filament.

Ca2+ 激活肌动蛋白-心肌肌球蛋白亚片段 1 MgATPase 速率显示细丝固有的协同作用。

• DOI: 10.1021/bi00376a022
• 发表时间: 1987
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Tobacman,LS

What sperm can teach us about energy production.

• DOI: 10.1111/j.1439-0531.2012.02071.x
• 发表时间: 2012-08
• 期刊: Reproduction in domestic animals = Zuchthygiene
• 作者: Mukai C;Travis AJ
• 通讯作者: Travis AJ