INFRASTRUCTURE 1: CORE RESEARCH LABORATORIES

基础设施 1：核心研究实验室

• 批准号: 8357141
• 负责人: Cesar Danilo Fermin
• 金额: $ 30.61万
• 依托单位: TUSKEGEE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357141
• 关键词: Animals; Biology; Biomedical Research; Cell Culture Techniques; Cell Separation; Cells; Chemistry; Computer software; Computers; Coupled; Digital Photography; Discipline; Equipment; Faculty; Flow Cytometry; Fluorescence Microscopy; Fluorescent Probes; Funding; Gel; Grant; Hour; Image; Image Analysis; Imagery; Laboratories; Laboratory Research; Life; Methodology; Microscope; Molecular Biology; National Center for Research Resources; Plants; Plug-in; Principal Investigator; Process; Recruitment Activity; Reindeer; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Running; Science; Series; Services; Source; Specimen; System; Time; United States National Institutes of Health; Universities; Veterinary Medicine; Visit; college; cost; digital; digital imaging; fluorescence microscope; fovea centralis; image processing; instrument; man; operation; prospective; research study; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The services and methodologies provided to the Tuskegee University research faculty by the CORE I Research Laboratories significantly enhance their research capabilities. Current and prospective users, about 17 (the number varies as new faculty are recruited), represent 3 academic colleges and diverse scientific disciplines. 1. Imaging Facility: The facility provides EM (the three instruments are being evaluated and consolidation is being considered, see discussion of Mr. Limbach's visit above), LM and gel imaging and analysis. Digital images are captured with a Kodak 290 used as Photoshop plug in or a Q Imaging CE camera operating through C Imaging Systems Simple PCI software (or as a Photoshop plug in) or MetaMorph software. Images are processed and/or analyzed with Photoshop using Fovea Pro 3 Image Analysis Tool Kit (Reindeer Graphics), or MetaMorph software. Gels are analyzed with Kodak EDAS 290 software. Operations are run on Macintosh or Gateway E Series (Windows XP) computers. Fluorescence microscopy, has been greatly enhanced by the addition of a Leica DM 5000B (upright microscope) coupled to the existing CE digital camera (operating through Photoshop,C Imaging or Simple PCI software). This system allows the visualization and digital capturing of images of specimens tagged with multiple fluorescent probes and the fusion of the multiple exposures into a single image. A Leica DM IRE2 (inverted fluorescent microscope) equipped with an incubation chamber has been acquired. This system is equipped with a similar digital photography system and will allow the visualization of live cell experiments over extended time periods. These instruments were obtained with funds provided by CBR/RCMI and the NIH Export Grant (#3P20MD000195-05S1). Additionally, Dr. Clayton Yates obtained (purchased with non-RCMI funds) an Olympus Spinning Disk Confocal Microscope System. This system is also equipped with long term culture capabilities and compatible digital image acquisition, processing and analysis software. This facility is used by the greatest diversity of investigators and has provided services to researchers from many disciplines including: Animal Science, Plant Science, Biology, Chemistry, Veterinary Biomedical Sciences and Pathobiology, and Material Sciences. 2. Cell Sorting / Flow Cytometry Facility: The Center acquired an Accuri C6 Flow Cytometer System. This system is administered by Dr. Clayton Yates and is primarily used by investigators from Biology and Veterinary Medicine. 3. Cell Culture and Molecular Biology Laboratories: These facilities provide laboratory space and molecular biology equipment to CBR/RCMI and other biomedical investigators. These laboratories easily receive the most "man-hours" of use of any of the CBR/RCMI CORE Facilities. Drs. T. Turner and R. Troy, (formerly CBR/RCMI funded projects), M. Martinez, and C. Yates's (CBR/RCMI funded projects) research teams use the facilities and equipment daily.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 核心I研究实验室为塔斯基吉大学研究人员提供的服务和方法显著提高了他们的研究能力。目前和潜在的用户约有17个（随着新教师的招聘，人数有所不同），代表着3个学术学院和不同的科学学科。 1.成像设备：该机构提供EM（正在评估这三种仪器，并考虑进行合并，见上文关于Limbach先生访问的讨论）、LM和凝胶成像和分析。数码影像是使用柯达290作为Photoshop插件或Q Imaging CE相机通过C Imaging Systems Simple PCI软件（或作为Photoshop插件）或MetaMorph软件拍摄的。使用Photoshop使用Fovea Pro 3图像分析工具包（Reindeer Graphics）或MetaMorph软件处理和/或分析图像。用Kodak EDAS 290软件分析凝胶。操作在Macintosh或Gateway E系列（Windows XP）计算机上运行。通过将Leica DM 5000 B（正置显微镜）与现有的CE数码相机（通过Photoshop、C Imaging或Simple PCI软件操作）连接，荧光显微镜得到了极大的增强。该系统允许用多个荧光探针标记的标本的图像的可视化和数字捕获，以及将多个曝光融合成单个图像。已获得配备有孵育室的Leica DM IRE 2（倒置荧光显微镜）。该系统配备了类似的数字摄影系统，并将允许活细胞实验在延长的时间段内可视化。这些工具是由CBR/RCMI和NIH出口赠款（#3P20MD000195- 05 S1）提供的资金获得的。此外，克莱顿耶茨博士获得（购买非RCMI基金）奥林巴斯旋转圆盘共聚焦显微镜系统。该系统还配备了长期培养能力和兼容的数字图像采集、处理和分析软件。该设施由最多样化的研究人员使用，并为许多学科的研究人员提供服务，包括：动物科学，植物科学，生物学，化学，兽医生物医学科学和病理学以及材料科学。 2.细胞分选/流式细胞仪设备：中心购置了Accuri C6流式细胞仪系统。该系统由克莱顿耶茨博士管理，主要由生物学和兽医学研究人员使用。 3.细胞培养和分子生物学实验室：这些设施为CBR/RCMI和其他生物医学研究人员提供实验室空间和分子生物学设备。这些实验室很容易获得CBR/RCMI核心设施中最多的“工时”。T医生Turner和R.特洛伊，（以前的CBR/RCMI资助的项目），M。Martinez和C.耶茨的（CBR/RCMI资助的项目）研究团队每天使用这些设施和设备。