INFRASTRUCTURE 1: CORE RESEARCH LABORATORIES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 核心研究实验室向塔斯基吉大学研究人员提供的服务和方法大大提高了他们的研究能力。目前和未来的用户约为17人(人数随着新教师的招聘而变化)，代表3所学术学院和不同的科学学科。 1.成像设施：该设施提供EM(三种仪器正在评估中，正在考虑合并，见上文关于林巴赫先生访问的讨论)、LM和凝胶成像和分析。数码图像是通过用作Photoshop插件的柯达290或通过C Image Systems Simple PCI软件(或作为Photoshop插件)或Metamorph软件运行的Q Image CE相机拍摄的。使用Fovea Pro 3图像分析工具包(驯鹿图形)或Metamorph软件，使用Photoshop处理和/或分析图像。凝胶用柯达EDAS 290软件进行分析。操作在Macintosh或Gateway E系列(Windows XP)计算机上运行。通过将徕卡DM 5000B(直立式显微镜)与现有CE数码相机(通过Photoshop、C Image或Simple PCI软件进行操作)相结合，大大增强了荧光显微镜的性能。该系统允许对标记有多个荧光探针的标本的图像进行可视化和数字捕获，并将多次曝光融合为一张图像。研制了一台配有孵化室的徕卡DM IRE2倒置荧光显微镜。该系统配备了类似的数字摄影系统，将允许在更长的时间段内可视化活细胞实验。这些工具是在CBR/RCMI和NIH出口赠款(#3P20MD000195-05S1)提供的资金下获得的。此外，克莱顿·耶茨博士还获得了(用非RCMI资金购买的)奥林巴斯旋转圆盘共聚焦显微镜系统。该系统还配备了长期的文化能力和兼容的数字图像采集、处理和分析软件。该设施由最多样化的研究人员使用，并为来自多个学科的研究人员提供服务，包括：动物科学、植物科学、生物学、化学、兽医生物医学科学和病理生物学以及材料科学。 2.细胞分选/流式细胞仪设施：该中心购置了Accuri C6流式细胞仪系统。该系统由克莱顿·耶茨博士管理，主要由生物学和兽医的研究人员使用。 3.细胞培养和分子生物学实验室：这些设施为CBR/RCMI和其他生物医学研究人员提供实验室空间和分子生物学设备。在CBR/RCMI的任何核心设施中，这些实验室很容易获得最多的“工时”。T.Turner博士和R.Troy博士(前CBR/RCMI资助的项目)、M.Martinez博士和C.Yates的(CBR/RCMI资助的项目)研究团队每天使用这些设施和设备。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The services and methodologies provided to the Tuskegee University research faculty by the CORE I Research Laboratories significantly enhance their research capabilities. Current and prospective users, about 17 (the number varies as new faculty are recruited), represent 3 academic colleges and diverse scientific disciplines. 1. Imaging Facility: The facility provides EM (the three instruments are being evaluated and consolidation is being considered, see discussion of Mr. Limbach's visit above), LM and gel imaging and analysis. Digital images are captured with a Kodak 290 used as Photoshop plug in or a Q Imaging CE camera operating through C Imaging Systems Simple PCI software (or as a Photoshop plug in) or MetaMorph software. Images are processed and/or analyzed with Photoshop using Fovea Pro 3 Image Analysis Tool Kit (Reindeer Graphics), or MetaMorph software. Gels are analyzed with Kodak EDAS 290 software. Operations are run on Macintosh or Gateway E Series (Windows XP) computers. Fluorescence microscopy, has been greatly enhanced by the addition of a Leica DM 5000B (upright microscope) coupled to the existing CE digital camera (operating through Photoshop,C Imaging or Simple PCI software). This system allows the visualization and digital capturing of images of specimens tagged with multiple fluorescent probes and the fusion of the multiple exposures into a single image. A Leica DM IRE2 (inverted fluorescent microscope) equipped with an incubation chamber has been acquired. This system is equipped with a similar digital photography system and will allow the visualization of live cell experiments over extended time periods. These instruments were obtained with funds provided by CBR/RCMI and the NIH Export Grant (#3P20MD000195-05S1). Additionally, Dr. Clayton Yates obtained (purchased with non-RCMI funds) an Olympus Spinning Disk Confocal Microscope System. This system is also equipped with long term culture capabilities and compatible digital image acquisition, processing and analysis software. This facility is used by the greatest diversity of investigators and has provided services to researchers from many disciplines including: Animal Science, Plant Science, Biology, Chemistry, Veterinary Biomedical Sciences and Pathobiology, and Material Sciences. 2. Cell Sorting / Flow Cytometry Facility: The Center acquired an Accuri C6 Flow Cytometer System. This system is administered by Dr. Clayton Yates and is primarily used by investigators from Biology and Veterinary Medicine. 3. Cell Culture and Molecular Biology Laboratories: These facilities provide laboratory space and molecular biology equipment to CBR/RCMI and other biomedical investigators. These laboratories easily receive the most "man-hours" of use of any of the CBR/RCMI CORE Facilities. Drs. T. Turner and R. Troy, (formerly CBR/RCMI funded projects), M. Martinez, and C. Yates's (CBR/RCMI funded projects) research teams use the facilities and equipment daily.