Tissue Procurement / Immunohistology

核心B（组织采购/免疫组织学）将作为既定组织和 研究病理学服务（TARPS）资源在UPCI。核心B将为所有四个项目提供支助 在这个项目中，它将采购、处理、分发或储存来自患有以下疾病的患者的标本： 癌症（口腔癌和其它HNC、黑色素瘤、前列腺癌或肾细胞癌）或对照个体， 例如，在一个实施例中，接受悬雍垂腭咽成形术或扁桃体切除术的非癌症患者作为HNC病例的对照。 标本，包括肿瘤活检以及肿瘤附近的正常组织，将从 由经过培训的人员根据既定SOP和以下条件处理患者或对照 严格的质量控制措施。将根据与以下人员建立的方案对样本进行分类： 参与研究者，并将伴随组织病理学诊断和临床/人口统计学 数据中心将向PPG研究者提供快速冷冻组织、冷冻切片， 常规石蜡包埋组织，塑料包埋和切片。核心B将提供常规和 特殊病理学服务，包括免疫组织化学、放射自显影、原位杂交、电子 显微镜、定量图像分析和形态测定以及数字成像和摄影。的 Core将处理新鲜或冷冻保存的标本，显微解剖组织并制备RNA或DNA作为1 needed.匹兹堡大学的石蜡组织微阵列（TMA）设施是 TARPS将提供给PPG研究者。还将根据需要制备组织微阵列。核心 B将提供核心数据库中研究中使用的每份标本的注释临床信息 并作为一个"诚实的经纪人"，以满足HIPAA的要求。援助将在 准备样本使用和访问相关临床信息的IRB申请。

Core B (Tissue Procurement/lmmunohistology) will operate as a part of the established Tissue and Research Pathology Services (TARPS) Resource at the UPCI. Core B will provide support to all four projects in this Program Project Grant. It will procure, process, and distribute or store specimens from patients with cancer (oral carcinoma and other HNC, melanoma, prostate or renal cell carcinomas) or control individuals, e.g., non-cancer patients undergoing uvulopalatopharyngoplasty ortonsillectomy as controls for HNC cases. Specimens, including tumor biopsies as well as normal tissues adjacent to tumors will be collected from patients or controls, processed by trained personnel in accordance with the established SOP and under rigorous quality control measures. Specimens will be triaged according to the protocol established with the participating investigators and will be accompanied by histopathologic diagnoses and clinical/demographic data. The Core will make available to the PPG investigators snap frozen tissues, frozen sections, conventional paraffin embedded tissues, plastic embedding and sectioning. Core B will provide routine and special pathology services including immunohistochemistry, autoradiography, in-situ hybridization, electron microscopy, quantitative image analysis and morphometry as well as digital imaging and photography. The Core will handle fresh or cryopreserved specimens, microdissect tissues and prepare RNA or DNA as1 needed. The Paraffin Tissue Micro-Array (TMA) facility of the University of Pittsburgh, which is a part of TARPS, will be available to the PPG investigators. Tissue microarrays will also be prepared as needed. Core B will provide annotated clinical information on every specimen used in research from the Core database and function as an "honest broker" to meet HIPAA requirements. Assistance will be available in the preparation of IRB applications for the specimen use and access to associated clinical information.