Tissue and Research Pathology Services

摘要：组织和研究病理学服务(TARPS) 组织和研究病理学服务(TARPS)是由CCSG资助的现有共享资源， 为利用UPMC患者的组织材料的UPCI研究项目提供中心支持。 问题资产救助计划的主要目标是提供一种机制，以简化、精简和标准化 研究组织的收益和支出过程，并提供有效的研究病理学支持 服务。不良资产救助计划的组织积累和支出职能起源于1991年，最初是一个小型服务机构， 重点关注泌尿生殖系癌症。在过去的23年里，资源有了很大的增长，现在 采购和发放各种癌症患者的组织材料，为 重大研究项目，如海德的卓越研究(孢子)专业计划和 颈部、肺癌、卵巢癌和皮肤癌；早期疾病研究网络(EDRN)相关的研究工作； 血液学-肿瘤学服务和其他几个癌症研究项目。不良资产救助计划的具体目标 1)提供组织和生物标本采购服务。这包括采购和 组织和生物标本的保存.标本的储存和归档及下游取回 为研究人员提供标本；提供具有适当质量的适当亚采样组织标本 样品的保证和质量控制；以及相关数据，根据项目的需要。2)提供 研究组织学服务。这包括组织样本的常规处理；设计和制造 石蜡组织微阵列(TMA)；新抗体的评估和建立方案； 使用自动化平台对常规和TMA组织切片进行免疫组织化学染色；以及 定量图像分析和形态测量服务，如有需要。3)用临床数据对样本进行注释。 数据注释是根据项目的需要进行的。不良资产救助计划利用资源，包括EMR、病理LIS和 UPMC癌症注册数据库，用于这些数据注释服务。4)提供完整的幻灯片成像和 图像分析服务。Tarp在TCGA计划中发挥了重要作用，提供了超过 1000箱。问题资产救助计划提供的服务包括：(1)组织和生物标本采购；(2) 研究组织学，(3)用临床数据对样本进行注释，以及(4)组织微阵列(TMA)设备(5) 定量图像分析和整体幻灯片成像。未来的努力包括开发一个大型阵列 从TCGA案例中提取TMA区块，以便于利用产生的分子数据。我们也是 参与了新协议的开发，以便能够在一张幻灯片上分析多个标记。 在本项目期间，所有10个研究项目的调查人员都使用了防水布。

Abstract: Tissue and Research Pathology Services (TARPS) The Tissue and Research Pathology Services (TARPS) is an existing CCSG-funded shared resource that serves as the central support for UPCI research programs that utilize tissue materials from UPMC patients. The main objectives of TARPS are to provide a mechanism to simplify, streamline, and standardize the process of research tissue accrual and disbursement, and to provide efficient research pathology support services. The tissue accrual and disbursement function of TARPS originated in 1991 as a small service facility, with a focus on genitourinary cancers. The resource has substantially expanded in the last 23 years and now procures and disburses tissue materials from patients with a diverse array of cancers, providing support to major research projects such as the Specialized Programs Of Research Excellence (SPORE) in Head and Neck, Lung, Ovary and Skin Cancers; the Early Disease Research Network (EDRN)-related research efforts; the Hematology-Oncology Service, and several other cancer research programs. The specific aims of TARPS are to: 1) Provide tissue and biological specimen procurement services. This includes procurement and preservation of tissue and biological specimens; storage and filing of specimens and downstream retrieval of specimens for researchers; providing appropriately sub-sampled tissue specimens with appropriate Quality assurance and Quality control of specimens; and associated data, as per the needs of the project. 2) Provide research histology services. This includes routine processing of tissue samples; design and manufacture paraffin tissue microarrays (TMAs); evaluation of novel antibodies and establishing protocols; immunohistochemical staining of conventional and TMA tissue sections, using an automated platform; and Quantitative Image Analysis and Morphometry services, when needed. 3) Annotate samples with clinical data. Data annotation is per needs of the project. TARPS utilizes resources including EMR, Pathology LIS and the UPMC Cancer Registry database for these data annotation services. 4) Provide whole slide imaging and image analysis services. TARPS has played a major role in the TCGA program having provided more than 1000 cases. Services provided by TARPS include: (1) tissue and biological specimen procurement, (2) research histology, (3) annotation of samples with clinical data, and (4) Tissue Micro-Array (TMA) facilities (5) Quantitative Image analysis and whole slide Imaging.. Future efforts include the development of a large array of TMA blocks from the TCGA cases to facilitate leveraging the molecular data generated. We are also engaged in the development of new protocols to enable profiling of numerous markers on a single slide. During the current project period investigators from all 10 Research Programs used TARPS.