University of Pittsburgh as the GUDMAP Tissue Hub and Collection Site

摘要 泌尿生殖道先天性疾病（肾、膀胱、输尿管、尿道等）是导致 器官衰竭伴随着增加的死亡风险，并且是日益增长的公共卫生负担。目前该 唯一的治疗方法是透析（用于肾脏）和器官移植。随着对移植的需求 供应过剩，迫切需要替代疗法。全面了解如何 泌尿生殖道在子宫内发育是有效开发新疗法以替代或修复 受伤的组织生殖泌尿系统发育分子解剖学项目（GUDMAP）已被 成功地提供了小鼠生殖泌尿系统基因表达的高分辨率图谱。 然而，人类泌尿生殖系统还没有类似的描述， 可能开发优化的实验技术来生长，扩增和分化人类泌尿生殖系统 体外祖细胞。发育生物学界的这些研究努力受到了阻碍 由于缺乏一个采购、质量控制和分配人类生殖泌尿系统药物的中心枢纽， 样品匹兹堡大学的健康科学组织库（HSTB）参与了人类 组织采购超过18年，具有悠久的收集，维护和分发历史 高质量的样本，研究科学家，无论是在内部和匹兹堡大学外。HSTB是 嵌入在匹兹堡卫生系统大学病理学系;从而提供 快速获取高质量的组织和生物标本。HSTB已建立同意协议 根据NIH的最佳实践建议，一个强大的信息学骨干，以促进 标本采购和注释，并有一个健全的质量控制和质量保证 程序. HSTB生物储存库得到了美国病理学家学院（CAP）的完全认证。HSTB 有一个既定的程序来获取胎儿组织胎儿组织IRB自2005年以来一直存在。HSTB 具有解剖标本和收集不同组织类型的基础设施。在这一年里，我们 已发放了从七十七宗个案收集的三百多个新样本。收藏品可以大幅增加 去年的案件多达725起，我们有初步数据 这表明我们可以从各种不同的组织中分离出人类泌尿生殖系统（肾脏、输尿管和膀胱）。 发育年龄（6-24周）。我们已经制作了这些泌尿生殖器官的出版质量图像 （包括肾脏和膀胱），并且还能够从各种泌尿生殖系统中分离和扩增细胞。 机关此外，我们已经将高质量的组织运送给各个GUDMAP研究者，他们已经验证了 组织的质量。我们建议作为GUDMAP组织中心和组织收集网站， 以HSTB现有的专业收集能力为基础，提供高质量的泌尿生殖系统服务。 向科学界成员提供样品，包括GUDMAP内部的成员。

ABSTRACT Congenital diseases of the genitourinary tract (kidneys, bladder, ureter, urethra etc.) are a leading cause of organ failure carrying with it an increased risk of death, and are a growing public health burden. At present, the only therapies are dialysis (for the kidneys) and organ transplantation. With the demand for transplants far exceeding supply there is an imminent need for alternate therapies. A comprehensive understanding of how the genitourinary tract develops in utero is necessary to effectively develop novel therapies to replace or repair injured tissue. The GenitoUrinary Development Molecular Anatomy Project (GUDMAP) has been successful at providing a high-resolution map of gene expression in the mouse GenitoUrinary system. However, a similar description has not been available for the human genitourinary system, nor has it been possible to develop optimized experimental techniques to grow, expand and differentiate human genitourinary progenitor cells in vitro. These research efforts by the developmental biology community have been hampered by the lack of a central hub for the procurement, quality control and distribution of human genitourinary samples. The Health Sciences Tissue Bank (HSTB) at the University of Pittsburgh has been involved in human tissue procurement for over 18 years, with a long standing history of collecting, maintaining and disbursing quality samples to research scientists, both in house and outside the University of Pittsburgh. HSTB is embedded within the Department of Pathology of the University of Pittsburgh Health Systems; thus providing rapid access to very high quality tissue and biological specimens. HSTB has established consenting protocols in line with the best practices recommendations from the NIH, a strong informatics backbone to facilitate specimen procurement and annotation, and has in place a robust quality control and quality assurance programs. The HSTB biorepository is fully accredited by the College of American Pathologists (CAP). HSTB has an established program accruing fetal tissues. The fetal tissue IRB has been in place since 2005. HSTB has the infrastructure for dissecting specimens and collecting different tissue types. In this calendar year, we have disbursed over 300 fresh samples collected from 77 cases. The collections can be significantly ramped up as material could have been accrued from as many as 725 cases last year. We have preliminary data showing that we can isolate the human urogenital system (kidneys, ureters and bladders) from various developmental ages (6-24 weeks). We have produced publication quality images of these genitourinary organs (including kidneys and bladder) and have also been able to isolate and expand cells from various genitourinary organs. Further, we have shipped high quality tissue to various GUDMAP investigators and they have verified the quality of the tissue sent. We propose to act as both the GUDMAP Tissue Hub and Tissue Gathering site to build upon the pre-existing specialized collecting abilities of HSTB and provide high quality genitourinary samples to members of the scientific community including those within GUDMAP.