University of Pittsburgh as the GUDMAP Tissue Hub and Collection Site

摘要 先天性泌尿生殖系统疾病(肾脏、膀胱、输尿管、尿路等)是一个主要原因 器官衰竭增加了死亡的风险，并成为日益严重的公共卫生负担。目前， 唯一的治疗方法是透析(肾脏)和器官移植。由于对移植的需求很大 供过于求，迫切需要替代疗法。全面了解如何 在子宫内发育的泌尿生殖道是有效开发替代或修复的新疗法所必需的。 受伤的组织。泌尿生殖系统发育分子解剖学项目(GUDMAP)已经 成功地提供了小鼠泌尿生殖系统基因表达的高分辨率图谱。 然而，人类的泌尿生殖系统还没有类似的描述，也没有。 有可能开发优化的实验技术来培养、扩大和分化人类生殖系统 体外培养的祖细胞。发育生物界的这些研究努力受到了阻碍。 由于缺乏人类生殖器的采购、质量控制和分配的中央中心 样本。匹兹堡大学的健康科学组织库(HSTB)参与了人类 纸巾采购超过18年，在收集、维护和支付方面有着悠久的历史 向匹兹堡大学内外的研究科学家提供高质量的样本。HSTB是 嵌入匹兹堡大学卫生系统大学病理学系；因此提供 快速获取非常高质量的组织和生物标本。HSTB已制定同意协议 根据美国国立卫生研究院的最佳实践建议，强大的信息学骨干力量为 样品采购和注释，并建立了强有力的质量控制和质量保证 程序。HSTB生物资料库获得了美国病理学家学会(CAP)的完全认可。HSTB 有一个既定的计划来积累胎儿组织。胎儿组织IRB自2005年以来一直存在。HSTB 拥有解剖标本和收集不同组织类型的基础设施。在这个历年，我们 已经发放了从77例病例中收集的300多个新鲜样本。收藏品的数量可以大幅增加 作为材料，去年可能有多达725起案件积累起来。我们有初步数据 表明我们可以将人类的泌尿生殖系统(肾脏、输尿管和膀胱)从不同的 发育年龄(6-24周)。我们制作了这些泌尿生殖器官的出版质量的图像 (包括肾脏和膀胱)，还能够分离和扩增各种泌尿生殖系统的细胞 器官。此外，我们已经将高质量的组织运送给GUDMAP的各种调查人员，他们已经核实了 送来的组织的质量。我们建议同时作为GUDMAP组织中心和组织收集站点来 建立在HSTB现有的专业采集能力基础上，提供高质量的生殖器 将样本提供给科学界成员，包括GUDMAP内的成员。

ABSTRACT Congenital diseases of the genitourinary tract (kidneys, bladder, ureter, urethra etc.) are a leading cause of organ failure carrying with it an increased risk of death, and are a growing public health burden. At present, the only therapies are dialysis (for the kidneys) and organ transplantation. With the demand for transplants far exceeding supply there is an imminent need for alternate therapies. A comprehensive understanding of how the genitourinary tract develops in utero is necessary to effectively develop novel therapies to replace or repair injured tissue. The GenitoUrinary Development Molecular Anatomy Project (GUDMAP) has been successful at providing a high-resolution map of gene expression in the mouse GenitoUrinary system. However, a similar description has not been available for the human genitourinary system, nor has it been possible to develop optimized experimental techniques to grow, expand and differentiate human genitourinary progenitor cells in vitro. These research efforts by the developmental biology community have been hampered by the lack of a central hub for the procurement, quality control and distribution of human genitourinary samples. The Health Sciences Tissue Bank (HSTB) at the University of Pittsburgh has been involved in human tissue procurement for over 18 years, with a long standing history of collecting, maintaining and disbursing quality samples to research scientists, both in house and outside the University of Pittsburgh. HSTB is embedded within the Department of Pathology of the University of Pittsburgh Health Systems; thus providing rapid access to very high quality tissue and biological specimens. HSTB has established consenting protocols in line with the best practices recommendations from the NIH, a strong informatics backbone to facilitate specimen procurement and annotation, and has in place a robust quality control and quality assurance programs. The HSTB biorepository is fully accredited by the College of American Pathologists (CAP). HSTB has an established program accruing fetal tissues. The fetal tissue IRB has been in place since 2005. HSTB has the infrastructure for dissecting specimens and collecting different tissue types. In this calendar year, we have disbursed over 300 fresh samples collected from 77 cases. The collections can be significantly ramped up as material could have been accrued from as many as 725 cases last year. We have preliminary data showing that we can isolate the human urogenital system (kidneys, ureters and bladders) from various developmental ages (6-24 weeks). We have produced publication quality images of these genitourinary organs (including kidneys and bladder) and have also been able to isolate and expand cells from various genitourinary organs. Further, we have shipped high quality tissue to various GUDMAP investigators and they have verified the quality of the tissue sent. We propose to act as both the GUDMAP Tissue Hub and Tissue Gathering site to build upon the pre-existing specialized collecting abilities of HSTB and provide high quality genitourinary samples to members of the scientific community including those within GUDMAP.