DEVELOPMENT AND VALIDATION OF STEROID AND OTHER ASSAYS BY LIQUID CHROMATOGRAPHY

类固醇和其他液相色谱分析方法的开发和验证

• 批准号: 8174528
• 负责人: CHRISTINA C. WANG
• 金额: $ 7.8万
• 依托单位: LUNDQUIST INSTITUTE FOR BIOMEDICAL INNOVATION AT HARBOR-UCLA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-01 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8174528
• 关键词: Androgens; Biological Assay; Blood; Blood Volume; Child; Clinical; Clinical Research; Computer Retrieval of Information on Scientific Projects Database; Detection; Development; Estradiol; Funding; Grant; Hormones; Human; Immunoassay; Institution; Laboratories; Liquid Chromatography; Measurement; Measures; Methods; Monitor; Ovary; Patients; Physicians; Postmenopause; Precocious Puberty; Research; Research Personnel; Resources; Sampling; Serum; Source; Steroids; Testing; Testosterone; United States National Institutes of Health; Validation; Woman; liquid chromatography mass spectrometry; steroid hormone; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. At present, current immunoassay methods cannot accurately or precisely measure low levels of steroid hormones in humans. As a result, below a certain lower limit of detection using conventional laboratory methods, testing for steroid levels in the blood often yields little clinically useful information. Postmenopausal women and women who have had their ovaries removed who are being studied for androgen replacement need an accurate method of monitoring treatment. Children who have clinical signs of precocious puberty need a method of measuring low testosterone or estradiol levels that may support clinical findings and are different from the levels of normal children. However, levels in normal children cannot be accurately measured because of the inherent problems of the current assay methods Tandem mass spectrometry (aka liquid chromatography-mass spectrometry-mass spectromery or LCMSMS) has the potential to sensitively, accurately, and precisely measure low levels of hormones. The Harbor-UCLA General Clinical Research Center would like to validate measurement of androgens, estradiol, steroid metabolites, and synthetic steroid compounds using tandem mass spectrometry. In order to validate measurements using tandem mass spectrometry, samples of human serum are needed. We propose to use leftover blood from patients whose clinical information is known and are having clinically indicated laboratory testing as ordered by his or her regular physicians. The volume of blood needed from each sample is approximately 200 ul.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 目前，当前的免疫测定方法无法准确或精确地测量人类中低水平的类固醇激素。 结果，使用常规实验室方法以下检测的一定下限，对血液中类固醇水平的测试通常几乎没有临床上有用的信息。 已去除卵巢的绝经后妇女和正在研究雄激素替代的妇女需要一种准确的监测治疗方法。 具有早熟青春期临床迹象的儿童需要一种测量低睾丸激素或雌二醇水平的方法，该方法可能支持临床发现，并且与正常儿童的水平不同。 但是，由于当前测定方法的固有问题，正常儿童的水平无法准确测量 串联质谱法（又称液相色谱 - 质谱质量质量光谱群或LCMSMS）具有敏感，准确和精确测量低水平的激素的潜力。 Harbour-UCLA一般临床研究中心希望使用串联质谱法验证对雄激素，雌二醇，类固醇代谢产物和合成类固醇化合物的测量。 为了使用串联质谱验证测量结果，需要人血清的样品。 我们建议使用已知临床信息的患者的剩余血液，并通过其常规医生进行了临床指示的实验室测试。 每个样品所需的血量约为200 ul。