DEVELOPMENT AND VALIDATION OF STEROID AND OTHER ASSAYS BY LIQUID CHROMATOGRAPHY
类固醇和其他液相色谱分析方法的开发和验证
基本信息
- 批准号:8174528
- 负责人:
- 金额:$ 7.8万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-12-01 至 2010-11-30
- 项目状态:已结题
- 来源:
- 关键词:AndrogensBiological AssayBloodBlood VolumeChildClinicalClinical ResearchComputer Retrieval of Information on Scientific Projects DatabaseDetectionDevelopmentEstradiolFundingGrantHormonesHumanImmunoassayInstitutionLaboratoriesLiquid ChromatographyMeasurementMeasuresMethodsMonitorOvaryPatientsPhysiciansPostmenopausePrecocious PubertyResearchResearch PersonnelResourcesSamplingSerumSourceSteroidsTestingTestosteroneUnited States National Institutes of HealthValidationWomanliquid chromatography mass spectrometrysteroid hormonetandem mass spectrometry
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
At present, current immunoassay methods cannot accurately or precisely measure low levels of steroid hormones in humans. As a result, below a certain lower limit of detection using conventional laboratory methods, testing for steroid levels in the blood often yields little clinically useful information. Postmenopausal women and women who have had their ovaries removed who are being studied for androgen replacement need an accurate method of monitoring treatment. Children who have clinical signs of precocious puberty need a method of measuring low testosterone or estradiol levels that may support clinical findings and are different from the levels of normal children. However, levels in normal children cannot be accurately measured because of the inherent problems of the current assay methods
Tandem mass spectrometry (aka liquid chromatography-mass spectrometry-mass spectromery or LCMSMS) has the potential to sensitively, accurately, and precisely measure low levels of hormones. The Harbor-UCLA General Clinical Research Center would like to validate measurement of androgens, estradiol, steroid metabolites, and synthetic steroid compounds using tandem mass spectrometry.
In order to validate measurements using tandem mass spectrometry, samples of human serum are needed. We propose to use leftover blood from patients whose clinical information is known and are having clinically indicated laboratory testing as ordered by his or her regular physicians. The volume of blood needed from each sample is approximately 200 ul.
这个子项目是许多研究子项目中的一个
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得了主要资金,
因此可以在其他CRISP条目中表示。所列机构为
研究中心,而研究中心不一定是研究者所在的机构。
目前,目前的免疫测定方法不能准确或精确地测量人体中低水平的类固醇激素。 因此,在使用传统实验室方法的一定检测下限以下,血液中类固醇水平的检测通常不会产生多少临床有用的信息。 绝经后妇女和正在接受雄激素替代治疗的卵巢切除妇女需要一种准确的监测治疗方法。 有性早熟临床症状的儿童需要一种测量低睾酮或雌二醇水平的方法,这种方法可能支持临床发现,并且与正常儿童的水平不同。 然而,由于目前测定方法的固有问题,不能准确测量正常儿童的水平
串联质谱法(又名液相色谱-质谱-质谱或LCMSMS)具有灵敏,准确和精确测量低水平激素的潜力。 Harbor-UCLA综合临床研究中心希望验证使用串联质谱法测量雄激素、雌二醇、类固醇代谢物和合成类固醇化合物。
为了验证使用串联质谱法的测量,需要人血清样品。 我们建议使用患者的剩余血液,这些患者的临床信息是已知的,并且正在按照其常规医生的要求进行临床指示的实验室检测。 每个样本所需的血液体积约为200 μ l。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
CHRISTINA C. WANG其他文献
CHRISTINA C. WANG的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('CHRISTINA C. WANG', 18)}}的其他基金
Community Outreach and Education Core (Core B)
社区外展和教育核心(核心 B)
- 批准号:
10018081 - 财政年份:2019
- 资助金额:
$ 7.8万 - 项目类别:
Community Outreach and Education Core (Core B)
社区外展和教育核心(核心 B)
- 批准号:
10615594 - 财政年份:2019
- 资助金额:
$ 7.8万 - 项目类别:
Community Outreach and Education Core (Core B)
社区外展和教育核心(核心 B)
- 批准号:
10260437 - 财政年份:2019
- 资助金额:
$ 7.8万 - 项目类别:
HYPOPITUITARISM IN RETIRED PROFESSIONAL FOOTBALL PLAYERS
退役职业足球运动员的垂体功能低下
- 批准号:
8174513 - 财政年份:2009
- 资助金额:
$ 7.8万 - 项目类别:
CLINICAL TRIAL: TRIAL TO EVALUATE THE EFFECTIVENESS AND SAFETY OF DIFF DOSES OF
临床试验:评估不同剂量的有效性和安全性的试验
- 批准号:
8174509 - 财政年份:2009
- 资助金额:
$ 7.8万 - 项目类别:
CLINICAL TRIAL: A MULTIPLE DOSE, PHARMACOKINETIC STUDY
临床试验:多剂量药代动力学研究
- 批准号:
8174500 - 财政年份:2009
- 资助金额:
$ 7.8万 - 项目类别:
A MULTICENTER, RANDOMIZED COMPARATOR TRIAL OF THE SAFETY AND SPERM AND GONADOTRO
安全性与精子和促性腺激素的多中心、随机比较试验
- 批准号:
8174515 - 财政年份:2009
- 资助金额:
$ 7.8万 - 项目类别:
相似海外基金
Establishment of a new biological assay using Hydra nematocyst deployment
利用水螅刺丝囊部署建立新的生物测定方法
- 批准号:
520728-2017 - 财政年份:2017
- 资助金额:
$ 7.8万 - 项目类别:
University Undergraduate Student Research Awards
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
10368760 - 财政年份:2017
- 资助金额:
$ 7.8万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
10669539 - 财政年份:2017
- 资助金额:
$ 7.8万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
9570142 - 财政年份:2017
- 资助金额:
$ 7.8万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
9915803 - 财政年份:2017
- 资助金额:
$ 7.8万 - 项目类别:
COVID-19 Supplemental work: POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER).
COVID-19 补充工作:用于确定组织特异性吸收电离辐射剂量的护理点生物测定(生物剂量计)。
- 批准号:
10259999 - 财政年份:2017
- 资助金额:
$ 7.8万 - 项目类别:
Drug discovery based on a new biological assay system using Yeast knock-out strain collection
基于使用酵母敲除菌株收集的新生物测定系统的药物发现
- 批准号:
21580130 - 财政年份:2009
- 资助金额:
$ 7.8万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Machine learning for automatic gene annotation using high-throughput biological assay data
使用高通量生物测定数据进行自动基因注释的机器学习
- 批准号:
300985-2004 - 财政年份:2005
- 资助金额:
$ 7.8万 - 项目类别:
Postdoctoral Fellowships
Machine learning for automatic gene annotation using high-throughput biological assay data
使用高通量生物测定数据进行自动基因注释的机器学习
- 批准号:
300985-2004 - 财政年份:2004
- 资助金额:
$ 7.8万 - 项目类别:
Postdoctoral Fellowships