MITOCHONDRIAL REDOX IMAGING OF COLON CANCER

结肠癌的线粒体氧化还原成像

• 批准号: 8169087
• 负责人: LIN LI
• 金额: $ 3.47万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169087
• 关键词: African American; American Indian and Alaska Native; Apoptosis; Asians; Colon Carcinoma; Computer Retrieval of Information on Scientific Projects Database; Death Rate; Flavoproteins; Funding; Grant; Hispanic Americans; Human; Image; Institution; Malignant Neoplasms; Metabolism; Mitochondria; Mus; Nicotinamide adenine dinucleotide; Oxidation-Reduction; Pacific Island Americans; Research; Research Personnel; Resources; Role; Scanning; Signal Transduction; Source; TP53 gene; Techniques; Tissues; United States National Institutes of Health; Woman; Xenograft procedure; base; cancer therapy; chemotherapy; clinical Diagnosis; cold temperature; fluorescence imaging; in vivo; melanoma; men

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Low temperature NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imaging or "redox scanning" has been developed to image the in vivo mitochondrial redox states of tissues on the basis of the redox ratios (Fp/NADH or Fp/(NADH+Fp)) and may be useful for clinical diagnosis and treatment of cancer given the central role of mitochondria in cellular metabolism, apoptosis and signaling transductions. Previously we have applied this technique to a panel of human melanoma mouse xenografts with different metastatic potential and identify Fp redox ratio correlates highly significantly with the aggressiveness of these melanomas. Colon cancer is the third most common cancer in US men and the second most common cancer in US women of Hispanic, American Indian/Alaska Native, or Asian/Pacific Islander ancestry, and the third most common cancer in white and African American women. Its death rate ranked third for both men and women in US. In this subproject we aim to achieve 1) redox imaging of colon cancer mouse xenografts with different status of p53; 2) redox imaging of colon cancer mouse xenografts receiving a chemotherapy; 3) study of the connection and interaction between p53 and mitochondrial redox state; 4) comparison of redox images between colon cancer with different aggressiveness.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 低温 NADH/Fp（还原型烟酰胺腺嘌呤二核苷酸/氧化黄素蛋白） 荧光成像或“氧化还原扫描”已被开发用于对体内线粒体进行成像 基于氧化还原比（Fp/NADH 或 Fp/(NADH+Fp)）的组织氧化还原状态，可以是 鉴于线粒体在癌症的临床诊断和治疗中的核心作用 细胞代谢、细胞凋亡和信号转导。之前我们已经应用过这个 具有不同转移潜能的一组人类黑色素瘤小鼠异种移植物的技术 并确定 Fp 氧化还原比与这些物质的攻击性高度显着相关 黑色素瘤。结肠癌是美国男性第三大常见癌症，也是第二大常见癌症 美国西班牙裔、美洲印第安人/阿拉斯加原住民或亚洲/太平洋裔女性的常见癌症 岛民血统，是白人和非裔美国女性中第三常见的癌症。 其死亡率在美国男性和女性中均排名第三。在这个子项目中，我们的目标是 实现1）不同p53状态的结肠癌小鼠异种移植物的氧化还原成像； 2) 接受化疗的结肠癌小鼠异种移植物的氧化还原成像； 3）研究 p53与线粒体氧化还原状态之间的联系和相互作用； 4）比较 具有不同侵袭性的结肠癌之间的氧化还原图像。