MITOCHONDRIAL REDOX IMAGING OF COLON CANCER

结肠癌的线粒体氧化还原成像

• 批准号: 8169087
• 负责人: LIN LI
• 金额: $ 3.47万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169087
• 关键词: African American; American Indian and Alaska Native; Apoptosis; Asians; Colon Carcinoma; Computer Retrieval of Information on Scientific Projects Database; Death Rate; Flavoproteins; Funding; Grant; Hispanic Americans; Human; Image; Institution; Malignant Neoplasms; Metabolism; Mitochondria; Mus; Nicotinamide adenine dinucleotide; Oxidation-Reduction; Pacific Island Americans; Research; Research Personnel; Resources; Role; Scanning; Signal Transduction; Source; TP53 gene; Techniques; Tissues; United States National Institutes of Health; Woman; Xenograft procedure; base; cancer therapy; chemotherapy; clinical Diagnosis; cold temperature; fluorescence imaging; in vivo; melanoma; men

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Low temperature NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imaging or "redox scanning" has been developed to image the in vivo mitochondrial redox states of tissues on the basis of the redox ratios (Fp/NADH or Fp/(NADH+Fp)) and may be useful for clinical diagnosis and treatment of cancer given the central role of mitochondria in cellular metabolism, apoptosis and signaling transductions. Previously we have applied this technique to a panel of human melanoma mouse xenografts with different metastatic potential and identify Fp redox ratio correlates highly significantly with the aggressiveness of these melanomas. Colon cancer is the third most common cancer in US men and the second most common cancer in US women of Hispanic, American Indian/Alaska Native, or Asian/Pacific Islander ancestry, and the third most common cancer in white and African American women. Its death rate ranked third for both men and women in US. In this subproject we aim to achieve 1) redox imaging of colon cancer mouse xenografts with different status of p53; 2) redox imaging of colon cancer mouse xenografts receiving a chemotherapy; 3) study of the connection and interaction between p53 and mitochondrial redox state; 4) comparison of redox images between colon cancer with different aggressiveness.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 低温NADH/FP（减少烟酰胺腺嘌呤二核苷酸/氧化黄素蛋白） 已经开发了荧光成像或“氧化还原扫描”以成像体内线粒体 基于氧化还原比（FP/NADH或FP/（NADH+FP））组织的氧化还原状态，可能是 鉴于线粒体在临床诊断和治疗中的临床诊断和治疗有用 细胞代谢，凋亡和信号转导。以前我们已经应用了 具有不同转移势的人类黑色素瘤小鼠异种移植的技术 并确定FP氧化还原比与这些比率高度显着相关 黑色素瘤。结肠癌是美国男性中第三大常见的癌症，第二大癌症 美国西班牙裔，美洲印第安人/阿拉斯加本地人或亚洲/太平洋的普通癌症 岛民血统，是白人和非裔美国妇女中第三大常见的癌症。 它的死亡率在美国的男性和女人中排名第三。在此副本中，我们的目标是 达到1）氧化癌小鼠异种移植物具有不同状态的氧化还原成像； 2）结肠癌小鼠异种移植物接受化学疗法的氧化还原成像； 3）研究 p53和线粒体氧化还原状态之间的连接和相互作用； 4）比较 与侵略性不同的结肠癌之间的氧化还原图像。