MITOCHONDRIAL REDOX IMAGING OF COLON CANCER

结肠癌的线粒体氧化还原成像

• 批准号: 8169087
• 负责人: LIN LI
• 金额: $ 3.47万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169087
• 关键词: African American; American Indian and Alaska Native; Apoptosis; Asians; Colon Carcinoma; Computer Retrieval of Information on Scientific Projects Database; Death Rate; Flavoproteins; Funding; Grant; Hispanic Americans; Human; Image; Institution; Malignant Neoplasms; Metabolism; Mitochondria; Mus; Nicotinamide adenine dinucleotide; Oxidation-Reduction; Pacific Island Americans; Research; Research Personnel; Resources; Role; Scanning; Signal Transduction; Source; TP53 gene; Techniques; Tissues; United States National Institutes of Health; Woman; Xenograft procedure; base; cancer therapy; chemotherapy; clinical Diagnosis; cold temperature; fluorescence imaging; in vivo; melanoma; men

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Low temperature NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imaging or "redox scanning" has been developed to image the in vivo mitochondrial redox states of tissues on the basis of the redox ratios (Fp/NADH or Fp/(NADH+Fp)) and may be useful for clinical diagnosis and treatment of cancer given the central role of mitochondria in cellular metabolism, apoptosis and signaling transductions. Previously we have applied this technique to a panel of human melanoma mouse xenografts with different metastatic potential and identify Fp redox ratio correlates highly significantly with the aggressiveness of these melanomas. Colon cancer is the third most common cancer in US men and the second most common cancer in US women of Hispanic, American Indian/Alaska Native, or Asian/Pacific Islander ancestry, and the third most common cancer in white and African American women. Its death rate ranked third for both men and women in US. In this subproject we aim to achieve 1) redox imaging of colon cancer mouse xenografts with different status of p53; 2) redox imaging of colon cancer mouse xenografts receiving a chemotherapy; 3) study of the connection and interaction between p53 and mitochondrial redox state; 4) comparison of redox images between colon cancer with different aggressiveness.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 低温NADH/Fp（还原型烟酰胺腺嘌呤二核苷酸/氧化型黄素蛋白） 已经开发了荧光成像或“氧化还原扫描”来对体内线粒体 基于氧化还原比（Fp/NADH或Fp/（NADH+Fp）），可以确定组织的氧化还原状态，并且可以 由于线粒体在癌症中的中心作用， 细胞代谢、凋亡和信号转导。此前，我们已将此 一组具有不同转移潜能人黑素瘤小鼠异种移植物的技术 并确定Fp氧化还原率与这些细胞的侵袭性高度显著相关， 黑素瘤。结肠癌是美国男性第三大常见癌症， 西班牙裔、美洲印第安人/阿拉斯加原住民或亚洲/太平洋裔美国女性的常见癌症 岛民血统，以及白色和非洲裔美国妇女的第三大常见癌症。 它的死亡率在美国男性和女性中排名第三。在这个子项目中，我们的目标是 实现1）p53不同状态结肠癌小鼠移植瘤的氧化还原成像; 2)接受化学疗法的结肠癌小鼠异种移植物的氧化还原成像; 3）对接受化学疗法的结肠癌小鼠异种移植物的氧化还原成像的研究。 p53与线粒体氧化还原状态之间的联系和相互作用; 不同侵袭性结肠癌之间的氧化还原图像。