MITOCHONDRIAL REDOX IMAGING OF COLON CANCER

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 低温NADH/FP(还原型烟酰胺腺嘌呤二核苷酸/氧化黄素蛋白) 荧光成像或“氧化还原扫描”已经被开发用来成像体内的线粒体 基于氧化还原比率(FP/NADH或FP/(NADH+FP))的组织的氧化还原状态，并且可以 鉴于线粒体在肿瘤中的核心作用，有助于癌症的临床诊断和治疗 细胞代谢、细胞凋亡和信号转导。以前我们已经应用了这一点 不同转移潜能的人黑色素瘤小鼠移植瘤的制作技术 确定FP氧化还原比率与这些疾病的侵袭性高度相关 黑色素瘤。结肠癌是美国男性第三常见的癌症，也是第二大常见癌症 拉美裔、美国印第安人/阿拉斯加原住民或亚洲/太平洋地区美国女性的常见癌症 岛民血统，白人和非裔美国女性中第三常见的癌症。 它的死亡率在美国男性和女性中都排名第三。在这个子项目中，我们的目标是 1)实现了不同状态p53基因的结肠癌小鼠移植瘤的氧化还原成像； 2)接受化疗的小鼠结肠癌移植瘤的氧化还原成像；3)研究 P53与线粒体氧化还原状态的联系和相互作用 不同侵袭性结肠癌之间的氧化还原图像。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Low temperature NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imaging or "redox scanning" has been developed to image the in vivo mitochondrial redox states of tissues on the basis of the redox ratios (Fp/NADH or Fp/(NADH+Fp)) and may be useful for clinical diagnosis and treatment of cancer given the central role of mitochondria in cellular metabolism, apoptosis and signaling transductions. Previously we have applied this technique to a panel of human melanoma mouse xenografts with different metastatic potential and identify Fp redox ratio correlates highly significantly with the aggressiveness of these melanomas. Colon cancer is the third most common cancer in US men and the second most common cancer in US women of Hispanic, American Indian/Alaska Native, or Asian/Pacific Islander ancestry, and the third most common cancer in white and African American women. Its death rate ranked third for both men and women in US. In this subproject we aim to achieve 1) redox imaging of colon cancer mouse xenografts with different status of p53; 2) redox imaging of colon cancer mouse xenografts receiving a chemotherapy; 3) study of the connection and interaction between p53 and mitochondrial redox state; 4) comparison of redox images between colon cancer with different aggressiveness.