OXIDATIVE POST-TRANSLATIONAL MODIFICATIONS IN CARDIOVASCULAR DISEASE

心血管疾病中的氧化翻译后修饰

• 批准号: 8170916
• 负责人: Catherine E. Costello
• 金额: $ 3.78万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170916
• 关键词: Bioinformatics; Biological; Blood Platelets; Cardiovascular Diseases; Clinical Investigator; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Computer software; Fourier Transform; Funding; Gel; Grant; High Pressure Liquid Chromatography; Institution; Laboratories; Methods; Modification; Oral; Oxidative Stress; Paper; Pathway interactions; Peptides; Performance; Post-Translational Protein Processing; Proteins; Proteolysis; Proteomics; Publishing; Reporting; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Whole Organism; computerized data processing; cryogenics; mass spectrometer; meetings; novel; posters; tissue/cell culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The research of the NHLBI-sponsored Cardiovasculaar Proteomics Center focuses on the oxidative post-translational modifications that occur in conjunction with cardiovascular disease. The Core laboratory develops appropriate methods and provides capLC-QTOF analysis of peptides in digests of 2D-gel separated or multistage HPLC purified proteins. In conjunction with the Resource, MALDI-TOF MS, LTQ-Orbitrap MS/MS and FTMS mass spectral analyses are also carried out. Software to assist in data processing and interpretation is being generated in the Center. A novel VC-MALDI cryogenic Fourier transform mass spectrometer with a 14-T magnet is being constructed to enable high throughput, high performance analysis of proteolysis mixtures from biological sources, and efficient, accurate determination of their post-translational modifications. Basic and clinical investigators, primarily located at BUSM and HMS, are using proteomic approaches to investigate the effects of oxidative stress on cell cultures, tissues and whole organisms. During this reporting year, in addition to numerous oral and poster meeting presentations, two methods papers that involved Resource collaboration, one on bioinformatics and one on novel hydrophobic fluorous derivatives, were published, in addition to applications papers that explored FTMS methods, and collaborations yielded papers on platelet stimulation pathways and oxidative modification of sirtuin.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 NHLBI赞助的心脏血管口蛋白质组学中心的研究重点是与心血管疾病结合进行的氧化后翻译后修饰。核心实验室开发了适当的方法，并提供了2D凝胶分离或多阶段HPLC纯化蛋白的消化中肽的CAPLC-QTOF分析。还与资源一起进行了MALDI-TOF MS，LTQ-ORBITRAP MS/MS和FTMS质谱分析。中心正在生成协助数据处理和解释的软件。正在构建一种具有14-T磁体的新型VC-Maldi低温傅立叶变换质谱仪，以实现来自生物学来源的蛋白水解混合物的高吞吐量，高性能分析，并有效，准确地确定其翻译后修饰。主要位于BUSM和HMS的基本和临床研究人员正在使用蛋白质组学方法来研究氧化应激对细胞培养物，组织和整个生物体的影响。在此报告年度，除了众多的口头和海报会议演讲外，还出版了两种涉及资源合作的方法论文，一种关于生物信息学和一种有关新型疏水性浮动衍生物的论文，除了探索FTMS方法的应用程序外，还发表了有关FTMS方法的应用，以及有关Sirtifatient of Sirturificative of Sirtiftifative of Sirteragicative of Sirtury of Sirterual of Sirterual of Sirturative of Sirtife of FTMS方法的作品。