ESTABLISHMENT OF SPECIFIC PATHOGEN FREE RHESUS MACAQUE COLONIES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 预计对印度恒河猴进行艾滋病相关研究的需求超过 从目前的国内育种计划中获得，迫切需要 为未来的艾滋病疫苗和发病机制研究扩大育种计划。恒河猴 具有确定的主要组织相容性复合体(MHC)基因类型的猕猴和 对于研究人员来说，已知的谱系变得越来越重要 宿主免疫反应的生物变异及其对疫苗策略和 艾滋病的发病机制。该项目的目标是扩大俄勒冈州 国家灵长类研究中心的无特定病原体(SPF)印度恒河猴 猕猴资源并充分鉴定它们的MHC单倍型 用于艾滋病研究的MHC I类等位基因的家系选育。特定的 实现这一目标的目标包括集中管理 该中心的SPF印度猕猴繁育群体，以最大限度地提高 遗传多样性的雌性，以扩大群体的繁殖能力。这个 繁育群体对10个MHC等位基因进行分型，并为生产已知亲缘关系的MHC定义的后代而进行管理。MHC型动物的选育和辅助繁殖技术都被用来提高未来的产量 MAMU-A*01和其他重要等位基因纯合的雄性育种者 用于评估猴免疫缺陷的病毒特异性细胞介导的免疫功能 预防艾滋病病毒感染的病毒疫苗模型。蜂群被维持在 最先进的野外避难所笼屋，由大约660个 成年和幼年动物，每年产生大约130只后代。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The projected need for Indian rhesus macaques for AIDS-related research exceeds availability from current domestic breeding programs and there is an urgent need to expand breeding programs for future AIDS vaccine and pathogenesis studies. Rhesus macaques with defined major histocompatability complex (MHC) genotypes and known pedigree are becoming increasingly important for research to understand biologic variation in host immune responses and its effects on vaccine strategies and the pathogenesis of AIDS. The objective of this project is to expand the Oregon National Primate Research Center's specific pathogen-free (SPF) Indian rhesus macaque resource and sufficiently characterize their MHC haplotype to permit selected pedigree breeding for MHC class I alleles useful in AIDS research. Specific aims for accomplishing this objective include intensively managing a subpopulation of the Center's SPF Indian rhesus macaque breeding colony to maximize production of genetically diverse females to expand the breeding capacity of the colony. The breeding colony is typed for ten MHC alleles and managed for the production of MHCdefined offspring of known parentage. Both selective breeding of MHC-typed animals and assisted reproduction technology are used to enhance production of future breeder males that are homozygous for the MAMU-A*01 and other alleles important for assessing virus-specific cell-mediated immune function in simian immunodeficiency virus vaccine models for preventing AIDS virus infection. The colony is maintained in state-of-the-art sheltered field cage housing and is comprised of approximately 660 adult and juvenile animals and produces approximately 130 offspring annually.