ESTABLISHMENT OF SPECIFIC PATHOGEN FREE RHESUS MACAQUE COLONIES

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 艾滋病相关研究对印度恒河猴的预计需求超过 目前国内育种计划的可用性，迫切需要 扩大未来艾滋病疫苗和发病机制研究的育种计划。恒河猴 具有确定的主要组织相容性复合体 (MHC) 基因型的猕猴 已知的血统对于研究了解变得越来越重要 宿主免疫反应的生物学变异及其对疫苗策略的影响 艾滋病的发病机制。该项目的目标是扩大俄勒冈州 国家灵长类研究中心的无特定病原体（SPF）印度恒河猴 猕猴资源并充分表征其 MHC 单倍型，以允许 为艾滋病研究中有用的 MHC I 类等位基因选择谱系育种。具体 实现这一目标的目标包括集中管理 该中心的 SPF 印度恒河猴繁殖群最大限度地提高了 基因多样化的雌性，以扩大群体的繁殖能力。的 育种群体针对 10 个 MHC 等位基因进行分型，并进行管理以产生已知亲本的 MHC 定义的后代。 MHC型动物的选育和辅助生殖技术都用于提高未来的产量 MAMU-A*01 和其他重要等位基因纯合的种公雄性 用于评估猿猴免疫缺陷病毒特异性细胞介导的免疫功能 用于预防艾滋病病毒感染的病毒疫苗模型。该菌落维持在 最先进的庇护野外笼舍，由大约 660 个 成年和幼年动物，每年产下约 130 只后代。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The projected need for Indian rhesus macaques for AIDS-related research exceeds availability from current domestic breeding programs and there is an urgent need to expand breeding programs for future AIDS vaccine and pathogenesis studies. Rhesus macaques with defined major histocompatability complex (MHC) genotypes and known pedigree are becoming increasingly important for research to understand biologic variation in host immune responses and its effects on vaccine strategies and the pathogenesis of AIDS. The objective of this project is to expand the Oregon National Primate Research Center's specific pathogen-free (SPF) Indian rhesus macaque resource and sufficiently characterize their MHC haplotype to permit selected pedigree breeding for MHC class I alleles useful in AIDS research. Specific aims for accomplishing this objective include intensively managing a subpopulation of the Center's SPF Indian rhesus macaque breeding colony to maximize production of genetically diverse females to expand the breeding capacity of the colony. The breeding colony is typed for ten MHC alleles and managed for the production of MHCdefined offspring of known parentage. Both selective breeding of MHC-typed animals and assisted reproduction technology are used to enhance production of future breeder males that are homozygous for the MAMU-A*01 and other alleles important for assessing virus-specific cell-mediated immune function in simian immunodeficiency virus vaccine models for preventing AIDS virus infection. The colony is maintained in state-of-the-art sheltered field cage housing and is comprised of approximately 660 adult and juvenile animals and produces approximately 130 offspring annually.