Binge Ethanol and Oocyte Quality

狂饮乙醇和卵母细胞质量

• 批准号: 7937105
• 负责人: CATHERINE A VANDEVOORT
• 金额: $ 58.64万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7937105
• 关键词: Acetaldehyde; Alcohol consumption; Animal Model; Behavior assessment; Blood; Cell physiology; Cells; Chromosomes; Detection; Development; Embryo; Embryonic Development; Embryonic and Fetal Development; Ethanol; Exposure to; Female; Fertility; Fertilization; Fertilization in Vitro; Fetal Growth; Fetus; Gene Expression; Goals; Growth; Growth and Development function; Health; Hormones; In Vitro; Incidence; Infant Development; Infant Health; Lipid Peroxidation; Luteal Phase; Macaca; Macaca mulatta; Measures; Modeling; Oocytes; Outcome; Ovarian; Partner in relationship; Pregnancy; Public Health; Reactive Oxygen Species; Schedule; Simulate; Structural Protein; Time; Training; Woman; aged; alcohol effect; alcohol exposure; base; binge drinking; blastocyst; granulosa cell; implantation; in vitro Model; in vivo; offspring; oocyte maturation; pregnant; preimplantation; prenatal; public health relevance; reproductive; reproductive function; reproductive hormone; research study; young adult

DESCRIPTION (provided by applicant): The overall goal of this project is to evaluate the effects of binge-type alcohol exposure on oocyte quality. There are no studies known to us that have examined the effects of binge alcohol consumption on fertility in women or any animal model, yet binge drinking is an increasing public health concern. In this project, young adult female rhesus monkeys would be trained to drink alcohol on a schedule that simulates binge drinking in women. The effects of at least 6 months of binge ethanol on oocyte health and ovarian function would be evaluated, including two cycles of superovlation to obtain oocytes for further study. Subsequently, females would be time-mated and as soon as pregnancy is detected, all ethanol consumption would be discontinued so that only the effects of ethanol on the oocytes and pre-implantation embryos, not the fetus, will be the focus of these studies. Hormone profiles, potential for oocyte development, oocyte and granulosa cell and gene expression, embryo development and fetal and infant development will be evaluated. Additionally, oocytes and granulosa cells will be exposed to ethanol in vitro to further study mechanisms of action. By utilizing the rhesus monkey, the outcome of these experiments will have direct relevance to the effects of binge drinking in women. PUBLIC HEALTH RELEVANCE: The incidence of binge drinking is increasing in young, reproductive aged women. There is little information available on the impact of this type of alcohol consumption on ovarian function, oocyte quality and subsequent embryo development and infant health. This project will investigate the effects and potential mechanisms of action of binge ethanol in young adult female rhesus monkeys, an excellent model for reproductive function. The outcome of this project will have direct relevance to the effects of binge drinking in women.

描述（由申请人提供）：该项目的总体目标是评估暴饮暴食型酒精暴露对卵母细胞质量的影响。据我们所知，目前还没有研究调查过酗酒对女性或任何动物生育能力的影响，但酗酒是一个日益严重的公共卫生问题。在这个项目中，年轻的成年雌性恒河猴将接受训练，按照模拟女性酗酒的时间表喝酒。研究人员将评估至少6个月暴饮乙醇对卵母细胞健康和卵巢功能的影响，包括两个周期的超排卵，以获得卵母细胞供进一步研究。随后，雌性将进行时间交配，一旦检测到怀孕，所有乙醇的消耗将停止，因此只有乙醇对卵母细胞和着床前胚胎的影响，而不是胎儿，将是这些研究的重点。将评估激素谱、卵母细胞发育潜力、卵母细胞和颗粒细胞及基因表达、胚胎发育以及胎儿和婴儿发育。此外，卵母细胞和颗粒细胞将在体外暴露于乙醇，以进一步研究其作用机制。通过使用恒河猴，这些实验的结果将与女性酗酒的影响直接相关。公共卫生相关性：在年轻的育龄妇女中，酗酒的发生率正在增加。关于这种类型的酒精消费对卵巢功能、卵母细胞质量以及随后的胚胎发育和婴儿健康的影响的信息很少。本项目将研究暴饮乙醇对年轻成年雌性恒河猴生殖功能的影响及其可能的作用机制。这个项目的结果将直接关系到酗酒对女性的影响。