DOSE SPARING OF TIV PROVIDED BY NP-ISS IN BABOONS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项在狒狒身上的研究将测试NP-ISS的能力，它包括与重组核蛋白(NP)结合的免疫刺激DNA序列(ISS)，以增强标准三价流感疫苗(TIV)的免疫原性，并可能允许节省剂量。 这项研究将由6组组成，每组5只动物，以测试以下各项： 仅TIV(45？g) 单独使用TIV(45？g)+NP-ISS(100？g) 仅TIV(15？g) 单独使用TIV(15？g)+NP-ISS(100？g) 仅TIV(5？g) 单独使用TIV(5？g)+NP-ISS(100？g) 疫苗配方将通过肌肉注射的方式在股四头肌注射。 NP-ISS之前曾在狒狒身上进行过测试。本实验将有助于确定NP-ISS是否可以增强对减少剂量TIV的体液免疫反应。这项研究可能对社会有益，因为它将有助于开发新的和改进的流感疫苗。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study in baboons will test the ability of NP-ISS, which consists of immunostimulatory DNA sequences (ISS) conjugated to recombinant nuclear protein (NP), to enhance the immunogenicity of the standard trivalent influenza vaccine (TIV) and possibly allow dose-sparing. The study will consist of 6 groups of 5 animals per group to test the following: TIV alone (45 ¿g) TIV alone (45 ¿g) + NP-ISS (100 ¿g) TIV alone (15 ¿g) TIV alone (15 ¿g) + NP-ISS (100 ¿g) TIV alone (5 ¿g) TIV alone (5 ¿g) + NP-ISS (100 ¿g) The vaccine formulations will be administered by intramuscular injection in the quadriceps. NP-ISS has been previously tested in baboons. This experiment will help determine if NP-ISS can enhance the humoral immune response to reduced doses of TIV. This study may be beneficial to society since it will help in the development of a new and improved influenza vaccine.