RHBMP-2 EFFECT ON BMD IN AN OSTEOPOROTIC BABOON VERTEBRAE + PROXIMAL FEMUR

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是评价rhBMP-2（一种骨诱导剂）是否能够改善骨质疏松狒狒椎体和股骨的骨量，从而降低骨脆性和骨折风险。我们建议给动物注射含有rhBMP-2的固体POE颗粒。手术过程将非常类似于椎体成形术，其中插管和荧光镜将用于识别椎体上的椎弓根，然后刺穿椎弓根，并注射POE/rhBMP-2颗粒。入口缺损用骨蜡封闭。同样的方法可以用于灵长类动物的股骨近端。作为研究的结果，我们预期观察到椎体和股骨近端的骨量增加。DEXA、microCT和组织学将是每个治疗组检查的参数。该项目可以解决日益增长的需要一种治疗，可以防止高风险个人的神经退行性骨折。目前旨在增加骨矿物质密度的全身疗法并不能消除估计的750，000例因骨质疏松症而发生的椎骨骨折。骨形态发生蛋白如rhBMP-2具有已知的局部骨形成记录，我们假设局部递送这种蛋白将成功地快速增强局部骨结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to evaluate whether rhBMP-2, a bone inducing agent, is capable of improving bone mass, thereby reducing bone fragility and fracture risk, in vertebral bodies and femurs of osteoporotic baboons. We propose injecting the animals with a solid POE pellet containing rhBMP-2. The surgical procedure would be very similar to a vertebroplasty surgery, where a cannula and a fluoroscope would be used to identify the pedicle on the vertebral body, the pedicle would then be pierced, and the POE/rhBMP-2 pellet injected. The entrance defect would be sealed with bone wax. The same approach can be utilized in the proximal femur of the primate. As a result of the study we would expect to observe an increased bone mass of the vertebral body and the proximal femur. DEXA, microCT and histology will be the parameters that will be examined per each treatment group. This project could address the growing need for a therapy that could prevent osteoporotic factures in high risk individuals. Current systemic therapies aimed at increasing bone mineral density do not eliminate the estimated 750,000 vertebral factures that occur as a result of osteoporosis. Bone morphogenetic proteins such as rhBMP-2 have a known record of local bone formation and we hypothesize that local delivery of this protein will be successful in rapidly enhancing local bone structure.