MITOCHONDRIAL REDOX IMAGING OF BIOLOGICAL TISSUES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 线粒体代谢功能成像的前沿技术进展 包括生物组织的氧化还原状态是非常需要的，因为线粒体及其 功能/遗传异常与包括癌症在内的许多疾病有关， 糖尿病、心血管疾病和神经退行性疾病。低温NADH/FP (还原烟酰胺腺嘌呤二核苷酸/氧化黄素蛋白)荧光成像或“氧化还原” 扫描“已经被开发出来，用来成像体内组织的线粒体氧化还原状态 氧化还原比率的基础(FP/NADH或FP/(NADH+FP))。基于ccd的氧化还原成像仪也已经被 为获得更快的图像和更高的空间分辨率(~10？m)而构建。然而，氧化还原比 是基于NADH和FP的荧光信号的相对强度之比， 通常取决于仪器设置，如过滤器和灯的条件。在这个子项目中，我们 目的1)对氧化还原扫描仪和CCD成像仪进行校准，以获得绝对NADH， FP和氧化还原比率，以方便比较不同时间和不同 仪器设置；2)氧化还原成像在鉴别乳腺肿瘤小鼠中的应用 具有不同转移潜能的异种移植瘤。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The development of cutting edge technologies for imaging the mitochondrial metabolic function including the redox state of biological tissues are in great need, since mitochondria and its functional/genetic abnormalities have been connected to a number of diseases including cancer, diabetes, cardiovascular diseases, and neurodegenerative diseases. Low temperature NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imaging or "redox scanning" has been developed to image the in vivo mitochondrial redox states of tissues on the basis of the redox ratios (Fp/NADH or Fp/(NADH+Fp)). A CCD-based redox imager has also been built to acquire images much faster with higher spatial resolution (~10 ¿m). However, redox ratio is based on the ratio of the relative intensities of the fluorescence signals of NADH and Fp, which usually depend on instrument settings such as filters and lamp conditions. In this subproject we aim to 1) perform calibrations of the redox scanner and the CCD imager to obtain absolute NADH, Fp and redox ratios to facilitate the comparison of results obtained at different time with different instrument settings; 2) application of redox imaging to differentiate breast tumor mouse xenografts with different metastatic potentials.