T1? & UTE MRI OF SHEEP STIFLES FOLLOWING SURGICALLY INDUCED MENISCAL TEAR

T1？

• 批准号: 8362016
• 负责人: Nicola FENTY
• 金额: $ 0.77万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362016
• 关键词: Applications Grants; Architecture; Biochemistry; Biomechanics; Cartilage; Control Animal; Data; Defect; Extramural Activities; Funding; Future; Grant; Healed; Histology; Image; Imaging Techniques; Knee; Lead; Limb structure; Magnetic Resonance; Magnetic Resonance Imaging; Mechanics; Meniscus structure of joint; Methodology; Modeling; National Center for Research Resources; Principal Investigator; Property; Research; Research Infrastructure; Resources; Sheep; Source; Testing; Thick; Time; Tissues; United States National Institutes of Health; Validation; X-Ray Computed Tomography; articular cartilage; cost; design; healing; nanofiber; novel; optical imaging; repaired; rho; scaffold

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Tears of the meniscus disrupt tissue architecture, impair mechanical function, and lead to the degeneration of knee cartilage. Current excisional treatments do not restore meniscal biomechanical properties. To that end, we have developed a novel biodegradable nanofibrous scaffold (NFS) to facilitate meniscus repair. Recently, we have begun testing these constructs in an ovine model, and are currently evaluating the meniscus healing, as well as the impact of the scaffold repair on the knee articular cartilage, via mechanical testing, histology, and biochemistry. In this study, we propose to utilize advanced imaging techniques to further evaluate the articular cartilage and menisci in knees with NFS-repaired menisci, as compared to control knees and knees with empty meniscus defects. For this, we propose to use two magnetic resonance imaging (MRI) sequences designed to look at the articular cartilage and menisci (T1-rho and ultrashort echo time (UTE), respectively), as well as micro-computed tomography (CT) to evaluate cartilage thickness. Limbs from study animals for control (n=1), empty defect (n=1) and NFS-repair (n=2) from the 6 month time point are now available for imaging. These data will provide important pilot information and validation of imaging methodologies to support inclusion of this approach in future grant applications to extramural agencies (i.e, NIH, DOD, VA).

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 半月板撕裂破坏组织结构，损害机械功能，并导致膝关节软骨退化。 目前的切除治疗不能恢复椎骨的生物力学特性。 为此，我们开发了一种新的可生物降解的纳米纤维支架（NFS），以促进半月板修复。 最近，我们已经开始在绵羊模型中测试这些结构，并且目前正在通过机械测试、组织学和生物化学评估半月板愈合以及支架修复对膝关节软骨的影响。 在这项研究中，我们建议利用先进的成像技术，以进一步评估关节软骨和半月板与NFS修复的半月板损伤的膝关节，与对照膝关节和膝关节空半月板缺损。 为此，我们建议使用两个磁共振成像（MRI）序列，旨在观察关节软骨和关节软骨（分别为T1-rho和超短回波时间（UTE）），以及微型计算机断层扫描（CT）来评估软骨厚度。 现在可对6个月时间点的对照（n=1）、空缺损（n=1）和NFS修复（n=2）研究动物的肢体进行成像。 这些数据将提供重要的试点信息和成像方法的验证，以支持将这种方法纳入未来向校外机构（即NIH，DOD，VA）的拨款申请中。