MRI TECHNIQUES FOR SMALL ANIMAL NEURO-IMAGING

小动物神经成像的 MRI 技术

• 批准号: 8361989
• 负责人: ARI BORTHAKUR
• 金额: $ 1.54万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361989
• 关键词: Alzheimer' s Disease; Animals; Biological Markers; Chemicals; Clinical; Diffusion; Early Diagnosis; Funding; Goals; Grant; Hippocampus (Brain); Histology; Human Resources; Image; Imaging Techniques; Immunohistochemistry; Magnetic Resonance; Magnetic Resonance Imaging; Measurement; Mus; National Center for Research Resources; Neurologic; Perfusion Weighted MRI; Principal Investigator; Protocols documentation; Research; Research Infrastructure; Resolution; Resources; Rodent; Scanning; Source; Spin Labels; Staging; Synapses; Tauopathies; Techniques; Training; Transgenic Mice; Translating; United States National Institutes of Health; cost; in vivo; mouse model; neuron loss; neuropathology; optical imaging; study characteristics; tau aggregation; treatment response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A transgenic mouse model (P301S, PS19 line) was recently developed for studying the characteristic tauopathy of Alzheimer's Disease (AD). Histological characterization of the neuropathology shows signs of synapse loss, hippocampal microgliosis, filamentous tau inclusions, and eventual neuron loss. The goal of this study is to characterize these mice non-invasively using magnetic resonance imaging (MRI), with the potential to translate these techniques into the clinical setting for early diagnosis of AD, as well as for tracking treatment response. A variety of MRI techniques may be suited for this task, including diffusion tensor MRI (DTI), high resolution MRI, arterial spin labeling (ASL) perfusion MRI, T1p MRI, and chemical exchange saturation transfer (CEST) imaging. This project is currently at an early stage of personnel training and preliminary scanning. In-vivo protocols for small rodent neurological imaging will need to be optimized. Resulting measurements and images will be compared with histology and immunohistochemistry characterization. It is our hope to provide widely accessible MRI techniques for small rodent imaging, as well as the discovery of non-invasive biomarkers for AD.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本研究建立了一种转基因小鼠模型（P301 S，PS19系）， 阿尔茨海默病（AD）的tau蛋白病。神经病理学的组织学特征显示 突触丢失、海马小胶质细胞增生、丝状tau内含物和最终神经元 损失本研究的目的是使用磁共振非侵入性地表征这些小鼠 磁共振成像（MRI），有可能将这些技术转化为临床环境， AD的诊断以及跟踪治疗反应。各种MRI技术可能是 适用于这项任务，包括扩散张量MRI（DTI），高分辨率MRI，动脉自旋标记 (ASL)灌注MRI、T1 p MRI和化学交换饱和转移（CEST）成像。这 项目目前正处于人员培训和初步扫描的早期阶段。体内 小型啮齿动物神经成像的方案需要优化。所得测量 并将图像与组织学和免疫组织化学表征进行比较。是我们 希望为小型啮齿动物成像提供广泛使用的MRI技术，以及发现 AD的非侵入性生物标志物。