APPLICATION OF NMR TO THE STUDY OF XYLOSE SYNTHASES

核磁共振在木糖合酶研究中的应用

• 批准号: 8361815
• 负责人: JOHN GLUSHKA
• 金额: $ 0.18万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361815
• 关键词: Animals; Bacteria; Enzymes; Eukaryota; Funding; Genes; Grant; Homologous Gene; NADH; National Center for Research Resources; Pentoses; Plants; Principal Investigator; Prokaryotic Cells; Ralstonia solanacearum; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; Uridine Diphosphate Glucuronic Acid; Uridine Diphosphate Sugars; Uridine Diphosphate Xylose; Xylose; apiose; cost; plant fungi

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The enzymes involved in converting UDP-GlcA to other UDP-sugars are well described in eukaryotes, but less is known in prokaryotes. Here we identify and characterize a gene (RsU4kpxs) from Ralstonia solanacearum str. GMI1000, which encodes a dual-function enzyme, UDP-4-keto-pentose/UDP-xylose synthase (UXS). One activity is to decarboxylate UDP-glucuronic acid to UDP-¿-L-threo-pentopyranosyl-4"-ulose (UDP-4-keto-pentose) in the presence of NAD+. The second converts UDP-4-keto-pentose and NADH to UDP-xylose and NAD+, albeit at a lower rate. The identification of the R. solanacearum enzyme enables us to propose that the ancestral UXS (as well as UDP-apiose synthase or UAXS) diverged into two distinct enzymatic activities in early bacteria. This separation gave rise to the current Uxs in animal, fungus and plant as well as to the plant Uaxs homologs.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 在真核生物中很好地描述了参与将UDP-GLCA转换为其他UDP-糖的酶，但在原核生物中已知鲜为人知。在这里，我们识别并表征了来自Ralstonia solanacearum str的基因（RSU4KPX）。 GMI1000，它编码双功能酶，UDP-4-酮五托蛋白/UDP-木糖合酶（UXS）。一种活性是在NAD+的存在下将二羧酸盐UDP-葡萄糖酸脱羧至UDP-thre-phreo-pentopyranosyl-4“ -Ulose（UDP-4-酮戊糖）。酶使我们能够提出祖先的UXS（以及UDP-植酶合酶或UAXS）在早期细菌中分解为两种不同的酶活性。