APPLICATION OF NMR TO THE STUDY OF XYLOSE SYNTHASES

核磁共振在木糖合酶研究中的应用

• 批准号: 8168895
• 负责人: JOHN GLUSHKA
• 金额: $ 0.17万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-10 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168895
• 关键词: Animals; Bacteria; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Eukaryota; Funding; Genes; Grant; Homologous Gene; Institution; NADH; Pentoses; Plants; Prokaryotic Cells; Ralstonia solanacearum; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Uridine Diphosphate Glucuronic Acid; Uridine Diphosphate Sugars; Uridine Diphosphate Xylose; Xylose; apiose; plant fungi

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The enzymes involved in converting UDP-GlcA to other UDP-sugars are well described in eukaryotes, but less is known in prokaryotes. Here we identify and characterize a gene (RsU4kpxs) from Ralstonia solanacearum str. GMI1000, which encodes a dual-function enzyme, UDP-4-keto-pentose/UDP-xylose synthase (UXS). One activity is to decarboxylate UDP-glucuronic acid to UDP-¿-L-threo-pentopyranosyl-4"-ulose (UDP-4-keto-pentose) in the presence of NAD+. The second converts UDP-4-keto-pentose and NADH to UDP-xylose and NAD+, albeit at a lower rate. The identification of the R. solanacearum enzyme enables us to propose that the ancestral UXS (as well as UDP-apiose synthase or UAXS) diverged into two distinct enzymatic activities in early bacteria. This separation gave rise to the current Uxs in animal, fungus and plant as well as to the plant Uaxs homologs.

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