INVESTIGATION OF UPTAKE & METABOLISM OF SIALIC ACID IN JURKAT CELLS

接受情况调查

• 批准号: 8361876
• 负责人: JOHN GLUSHKA
• 金额: $ 0.18万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361876
• 关键词: Carbon; Epitopes; Funding; Grant; Imagery; Investigation; Jurkat Cells; Label; Ligation; Metabolic; Metabolism; NMR Spectroscopy; National Center for Research Resources; Nitrogen; Polysaccharides; Principal Investigator; Research; Research Infrastructure; Resources; Sialic Acids; Source; United States National Institutes of Health; cost; imaging probe; sugar; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Cellular glycans can be metabolically labeled with azido sugars and thus further conjugated with useful imaging probes or epitope tags (e.g. Scott T Laughlin, Carolyn R Bertozzi, 2007, "Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation. ", Nat. Protoc. Vol 2, 2930-2944). To better understand the uptake of these compounds and the subsequent metabolic steps, we are exploring the use of nuclear magnetic resonance (NMR) spectroscopy to observe carbon-13 and nitrogen-15 isotopically enriched sugars such as sialic acid and N-azidoacetylmannosamine introduced into Jurkat cells.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 细胞聚糖可以用叠氮基糖进行代谢标记，并因此进一步与有用的成像探针或表位标签缀合（例如Scott T Laughlin，Carolyn R Bertozzi，2007，“Metabolic labeling of glycans with azido sugar and subsequent glycan-profiling and visualization via Staudinger ligation.“，Nat. Protoc. 2930-2944）。 为了更好地了解这些化合物的摄取和随后的代谢步骤，我们正在探索使用核磁共振（NMR）光谱来观察引入Jurkat细胞的碳-13和氮-15同位素富集的糖，如唾液酸和N-叠氮乙酰甘露糖胺。