INVESTIGATION OF UPTAKE & METABOLISM OF SIALIC ACID IN JURKAT CELLS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 细胞多聚糖可以被叠氮糖代谢标记，从而进一步与有用的成像探针或表位标签结合(例如Scott T Laughlin，Carolyn R Bertozzi，2007，“用叠氮糖代谢标记多聚糖和随后的多聚糖--通过Staudinger连接进行的糖谱分析和可视化”，NAT。普罗托克。第二卷，2930-2944)。为了更好地了解这些化合物的摄取和随后的代谢步骤，我们正在探索使用核磁共振(核磁共振)光谱来观察碳-13和氮-15同位素浓缩糖，如唾液酸和N-叠氮乙酰甘露糖胺引入Jurkat细胞。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Cellular glycans can be metabolically labeled with azido sugars and thus further conjugated with useful imaging probes or epitope tags (e.g. Scott T Laughlin, Carolyn R Bertozzi, 2007, "Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation. ", Nat. Protoc. Vol 2, 2930-2944). To better understand the uptake of these compounds and the subsequent metabolic steps, we are exploring the use of nuclear magnetic resonance (NMR) spectroscopy to observe carbon-13 and nitrogen-15 isotopically enriched sugars such as sialic acid and N-azidoacetylmannosamine introduced into Jurkat cells.