NMR STUDIES OF GLUCOSE METABOLISM IN CATS

猫葡萄糖代谢的核磁共振研究

• 批准号: 8361797
• 负责人: JOHN GLUSHKA
• 金额: $ 0.18万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361797
• 关键词: Algorithms; Carbon; Data; Deuterium; Felis catus; Funding; Glucose; Grant; Isotope Labeling; Label; Measures; Metabolic Pathway; National Center for Research Resources; Obesity; Plasma; Principal Investigator; Publishing; Relative (related person); Research; Research Infrastructure; Resources; Site; Source; United States National Institutes of Health; cost; glucose metabolism; propylene

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Glucose metabolism in cats under a variety of dietary conditions is being analyzed as part of a study of obesity. By following isotope labels (carbon-13 and deuterium) and measuring amounts of label at specific atom sites in glucose, one can evaluate different metabolic pathways. The analysis involves preparing and extracting a glucose derivative (1,2-isopropylidene-glucofuranose or 'monoacetone glucose') from plasma, collecting carbon-13 and deuterium NMR spectra of this compound, and measuring relative peak intensities. The data are then analyzed according to published algorithms to identify the source of each atom.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 作为肥胖研究的一部分，正在分析猫在各种饮食条件下的葡萄糖代谢。通过跟踪同位素标记（碳-13和氘）并测量葡萄糖中特定原子位点的标记量，可以评估不同的代谢途径。该分析涉及从血浆中制备和提取葡萄糖衍生物（1，2-异亚丙基-呋喃葡萄糖或“单丙酮葡萄糖”），收集该化合物的碳-13和氘NMR光谱，并测量相对峰强度。然后根据已发布的算法分析数据，以确定每个原子的来源。