NMR STUDIES OF GLUCOSE METABOLISM IN CATS

猫葡萄糖代谢的核磁共振研究

• 批准号: 7957527
• 负责人: JOHN GLUSHKA
• 金额: $ 0.22万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957527
• 关键词: Algorithms; Carbon; Computer Retrieval of Information on Scientific Projects Database; Data; Deuterium; Felis catus; Funding; Glucose; Grant; Human; Institution; Isotope Labeling; Isotopes; Label; Measures; Metabolic Pathway; Obesity; Plasma; Preparation; Protocols documentation; Publishing; Rattus; Relative (related person); Research; Research Personnel; Resources; Sampling; Signal Transduction; Site; Source; Testing; United States National Institutes of Health; Work; data acquisition; glucose metabolism; propylene

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Glucose metabolism in cats under a variety of dietary conditions is being analyzed as part of a study of obesity. By following isotope labels (carbon-13 and deuterium) and measuring amounts of label at specific atom sites in glucose, one can evaluate different metabolic pathways. The analysis involves preparing and extracting a glucose derivative (1,2-isopropylidene-glucofuranose or 'monoacetone glucose') from plasma, collecting carbon-13 and deuterium NMR spectra of this compound, and measuring relative peak intensities. The data are then analyzed according to published algorithms to identify the source of each atom. The collaborative work includes modifying sample preparation and NMR data acquisition protocols in order to increase overall signal sensitivity, due to the lower amounts of plasma and isotope enrichment available in the cat samples relative to published work on rats and humans. In addition to the medically relevant data, this project will be a test application of analytical NMR.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 作为肥胖研究的一部分，正在分析猫在各种饮食条件下的葡萄糖代谢。通过跟踪同位素标记（碳 13 和氘）并测量葡萄糖中特定原子位点的标记量，可以评估不同的代谢途径。该分析包括从血浆中制备和提取葡萄糖衍生物（1,2-异亚丙基-呋喃葡萄糖或“单丙酮葡萄糖”），收集该化合物的碳 13 和氘核磁共振谱，并测量相对峰强度。然后根据已发布的算法对数据进行分析，以确定每个原子的来源。 合作工作包括修改样品制备和核磁共振数据采集方案，以提高整体信号灵敏度，因为与已发表的大鼠和人类研究相比，猫样品中可用的血浆和同位素富集量较低。除了医学相关数据外，该项目还将是分析核磁共振的测试应用。