NMR STRUCTURAL ANALYSIS OF MAN10 AND MAN11/12

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Data from three samples were analyzed for the presence of beta-mannosyl residues. Sample 1: "man10" - this was the original sample described as a man9/10 mixture Sample 2: "man11" - this was a second preparation described as man11/man12 mixture from P4 fractions 63-66 with estimated 50% man11, 15% man12. Sample3: "man10_digest" - this was a third preparation wherein the original man11/man12 mixture was treated with alpha1,2- mannosidase to trim back to man9 or man10. A fourth sample containing the core man8/man9 mixture was also examined. Samples were lyophilized from D2O, and redissolved into 200 uL or 500 uL D2O. NMR data were collected on Varian Inova 600 MHz and 800 MHz spectrometers at 25¿C. Standard experiments from the Varian library (gradient COSY, TOCSY, NOESY, gradient HSQC and HMBC) were used for characterization.