HIGH RESOLUTION OPTICAL DIAGNOSTICS FOR ORAL AND LUNG CANCER

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 吸烟引起的支气管癌和口腔癌是烟草导致死亡的主要原因。需要更准确的诊断方法来提高治疗和存活率。我们正在开发高分辨率(接近组织学水平)灵活的光纤内窥镜光学相干断层扫描方法，用于口腔和气道癌的检测和监测。光学相干层析成像(OCT)和光学多普勒层析成像(OCT)使用干涉宽带相干选通反射技术来获得定义的高分辨率空间位置的高分辨率结构和毛细血管流动动力学，即使在口腔粘膜和呼吸道等高散射组织中也是如此。2-10um的OCT分辨率使我们能够开始研究体内组织结构和微循环的非侵入性光学成像，以诊断口腔和呼吸道癌症。 我们建议通过进一步开发用于肺部和口腔肿瘤学应用的柔性光纤OCT/ODT来改善吸烟引起的呼吸道和口腔癌的诊断和治疗。 具体目标： 1.建立实时、灵活的纤维支气管镜检查方法，对呼吸道和口腔肿瘤进行高分辨率的OCT/ODT检查。 2.评价这些OCT/ODT在活体动物模型中识别口腔癌和在吸烟诱导的呼吸道恶性肿瘤患者中识别气道癌的能力。 3.评估低分辨率体内肿瘤荧光定位与OCT/ODT高分辨率成像相结合的可能性，以提高口腔癌和气道癌的诊断效率和检测效率，并更好地了解恶变的功能光学标志物。 4.建立了体内超高分辨率钛宝石OCT成像方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Smoking induced endobronchial and oral cancers are leading causes of tobacco induced mortality. More accurate methods for diagnosis are needed to improve treatment and survival. We are developing-high resolution (near histological level) flexible fiberoptic endoscopic optical coherence tomographic methods for oral and airway cancer detection and monitoring. Optical coherence tomography (OCT) and optical Doppler tomography use interferometric broad-band coherent gating reflectance techniques to obtain high resolution structural and capillary flow dynamics at defined high resolution spatial locations even in highly scattering tissues like oral mucosa and airways. The 2-10 um OCT resolution capabilities enable us to begin investigation of non-invasive optical imaging of in-vivo tissue structure and microcirculation for diagnosis of oral and airway cancers. We propose to improve diagnosis and treatment of smoking induced airway and oral cancers by further development of flexible fiberoptic OCT/ODT for pulmonary and oral oncologic applications. Specific Aims: 1. To develop real-time flexible fiberoptic bronchoscopic methods for high-resolution simultaneous OCT/ODT evaluation of airway and oral tumors. 2. Evaluate these OCT/ODT capabilities for identifying oral cancers in live animal models and airway cancers in patients with smoking induced airway malignancy. 3. Assess the potential for combining low resolution in-vivo tumor fluorescence localization with OCT/ODT high resolution imaging to improve diagnostic yield and detection efficiency for oral and airway cancers, and to better understand functional optical markers for malignant transformation. 4. Develop methods for ultra-high resolution Ti:sapphire in-vivo OCT imaging.