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In vivo Imaging and Quantification of Cilia Beating Dynamics Using Phase-Resolved Optical Imaging Technology

使用相位分辨光学成像技术对纤毛跳动动力学进行体内成像和量化

基本信息

批准号：
10033635
负责人：
ZHONGPING CHEN
金额：
$ 59.36万
依托单位：
UNIVERSITY OF CALIFORNIA-IRVINE
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-01 至 2024-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10033635
关键词：
3-Dimensional Absenteeism Adult Allergic rhinitis American Antibiotics Asthma Biomedical Engineering Biophysics Blood Pressure Catheters Child Chronic Obstructive Airway Disease Cilia Clinical Collaborations Congestive Heart Failure Cystic Fibrosis Data Development Device or Instrument Development Devices Diagnosis Discipline Disease Drug Delivery Systems Drug Monitoring Drug usage Endoscopes Endoscopy Excision Failure Frequencies Functional Imaging Functional disorder General Anesthesia Health Health Expenditures Heart Rate Human Image Imaging Device Imaging technology In Vitro Industry Inflammatory Interferometry Investigation Knowledge Lasers Light Link Maps Measurement Measures Medical Methods Microscope Modeling Monitor Morbidity - disease rate Morphologic artifacts Motion Mucociliary Clearance Mucous Membrane National Institute of Biomedical Imaging and Bioengineering Noise Nose Operative Surgical Procedures Optical Coherence Tomography Optics Oryctolagus cuniculus Outcome Measure Parkinson Disease Patient Outcomes Assessments Patients Pattern Performance Performance at work Pharmaceutical Preparations Pharmacologic Substance Pharmacology Pharmacotherapy Phase Physicians Physiological Physiology Pilot Projects Population Prevalence Primary Ciliary Dyskinesias Property Publishing Quality of life Reporting Research Research Project Grants Resolution Respiration Disorders Respiratory Mucosa Scanning Scheme Schools Scientist Series Severity of illness Sinus Site Source Speed Standardization Structure Structure of mucous membrane of nose Surface Surveys Swab System Technology Temperature Testing Therapeutic Time Tissue Sample Tissue imaging Tissues Translating United States Visual Visualization Work allergic airway disease awake base body system burden of illness chronic back pain chronic rhinosinusitis clinical application clinical translation cost design design and construction drug development economic cost economic impact experience human subject imaging modality imaging system in vivo in vivo imaging in vivo imaging system innovation minimally invasive mortality operation optical imaging recruit research clinical testing response rhinosinusitis success treatment response virtual

项目摘要

PROJECT SUMMARY Each year, 50M Americans suffer from chronic rhinosinusitis (CRS) and allergic rhinitis (AR). The economic impact accounts for over $35B/year in health care expenditures alone, with over 3.5M work and 2M school days lost each year. Accordingly, over 600K operations per year are performed to treat sinonasal disease. The disease burden is immense with a profound reduction in quality of life, and is often overlooked because mortality is low, while morbidity is high. Currently, patient-reported outcome measures are used to evaluate disease status in CRS and AR, as there are no reliable quantitative methods to gauge disease severity or response to therapy. Success or failure is determined by the subjective reports from the patient alone, or via physician-directed endoscopy, where interpretation may also be subjective. One potential biophysical variable that can be measured is ciliary beat frequency (CBF). The cilia control mucociliary transport, which is the endpoint physiologic function of the sinonasal mucosa. CBF is challenging to measure in vivo in a clinical setting, but has value in potentially monitoring mucosal health and the response to therapy. This proposal is in response to the PAR-19-158 NIBIB Bioengineering Research Grants and aimed to develop and validate an innovative in vivo imaging system to measure CBF and the related physiological parameters that characterize mucosal health. Drs. Chen and Wong have had continuous collaborations for over 20 years with expertise in optical imaging, system, and probe designs, as well as translating these bench-top technologies to clinical applications. This proposal centers on the design, construction, and clinical evaluation of phase-resolved spectrally encoded endoscopy (PR-SEE) integrated with optical coherence tomography (OCT). PR-SEE will enable functional imaging of the nasal mucosa in vivo, allowing surveying the CBF landscape across the nasal mucosa as well as facilitating the analysis of ciliary beat pattern (CBP). More importantly, PR-SEE will provide a rigorous means to assess the speed, amplitude, and propagation of the mucosal metachronal waves (MWs), which are the quantifiable endpoint function of mucociliary transport. The proposed imaging device concentrates on a coaxial scanning scheme encompassing spectrally encoded interferometry and OCT to overcome the current limitations for in vivo cilia imaging. To evaluate and validate our device, we will first perform PR-SEE on a rabbit nasal airway model, followed by imaging of anesthetized patients in the operation room to obtain ciliary functional parameters (CBF, CBP, MWs). Sixty subjects undergoing nasal operations (normal control, e.g., septoplasty) or sinus surgeries (CRS patients) will be recruited for the study. Successful clinical translation in the operation room will prepare PR-SEE for imaging awake patients in the office in subsequent studies. We firmly believe that enabling functional quantification of mucosal health will jumpstart the developments in pharmacotherapy, devices, and surgical intervention.

项目总结