IMAGING INTERACTION OF STEM CELLS WITH BRAIN SLICE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our preliminary data establish that stem cells can migrate toward brain tumor microenvironment. We would like to visualize how stem cells migrate toward the brain tumor by using ex vivo brain slice model. Here, we will determine how stem cells behave in brain slice microenviornment by using confocal microscopy. Specifically, we will (1) Examine the migration of stem cells inside the brain slice (invasion into a deeper level) by 3-D reconstitution to visualize the migratory path; (2) Examine how the stem cells contact with the brain slice residential cells by neurite extension; and (3) Examine the effects of brain slice microenvironment on the differentiation of stem cells.