ACBT GLIOMA SPHEROIDS

ACBT 胶质瘤球体

• 批准号: 8362626
• 负责人: TATIANA B KRASIEVA
• 金额: $ 0.51万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362626
• 关键词: Acute; Apoptosis; Biological Assay; Biotechnology; Cell Line; Cell Survival; Cells; Cellular Spheroids; Chronic; Cytosol; Endocytosis; Endosomes; Fluorescence Microscopy; Funding; Gene Expression; Genes; Glioma; Grant; Image; In Vitro; Intracranial Neoplasms; Lasers; Life; Lysosomes; National Center for Research Resources; Photosensitizing Agents; Principal Investigator; Rattus; Reporter Genes; Research; Research Infrastructure; Resources; Site; Source; Therapeutic; Tumor Suppressor Genes; United States National Institutes of Health; base; cost; gene therapy; glioma cell line; in vivo; macromolecule; monolayer; multi-photon

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. ACBT spheroids following acute, chronic, and repetitive PDT will be imaged for live-dead assays and apoptosis. The therapeutic usefulness of gene therapy is often limited by an inefficient transfer of the macromolecule into the cytosol, and a lack of site-specific targeting. Photochemical internalization (PCI) is based on photochemically induced release of endocytosed macromolecules from endosomes and lysosomes into the cytosol. PCI has been used in combination with gene therapy and has been shown to increase gene gene expression. The ability of PCI to enhance the gene insertion of the tumor suppressor gene PAX6 or reporter genes into glioma cells will be studied in vivo and in vitro. Monolayer and multicellular spheroid cultures of the rat glioma cell lines F98 and RG2 will be used in the in vitro studies. The same cell lines will be used to induce intracranial tumors in rats. Confocal and multi photon fluorescence microscopy will be used to study photosensitizer distribution, expression of reporter gene (GFP), and cell survival.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 急性、慢性和重复PDT后的ACBT球状体将成像用于活-死测定和细胞凋亡。 基因治疗的治疗有效性通常受到大分子向胞质溶胶中的低效转移以及缺乏位点特异性靶向的限制。光化学内化（PCI）是基于光化学诱导的内吞大分子从内体和溶酶体释放到胞质溶胶中。PCI已与基因治疗联合使用，并已显示增加基因表达。将在体内和体外研究PCI增强肿瘤抑制基因PAX 6或报告基因插入胶质瘤细胞的能力。大鼠胶质瘤细胞系F98和RG 2的单层和多细胞球体培养物将用于体外研究。相同的细胞系将用于诱导大鼠颅内肿瘤。共聚焦和多光子荧光显微镜将用于研究光敏剂分布，报告基因（GFP）的表达和细胞存活。