ROLE OF SMC COMPLEXES IN DNA REPAIR

SMC 复合物在 DNA 修复中的作用

• 批准号: 8362704
• 负责人: TATIANA B KRASIEVA
• 金额: $ 0.51万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362704
• 关键词: Biotechnology; Chromatin; Collaborations; Complex; DNA Damage; DNA Repair; Funding; Future; Gene Expression Regulation; Grant; Kinetics; Laboratory Study; Lasers; Mitosis; Multiprotein Complexes; National Center for Research Resources; Principal Investigator; Regulation; Research; Research Infrastructure; Resources; Role; Site; Source; System; United States National Institutes of Health; cancer therapy; cost; insight; therapeutic target

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. SMC complexes are conserved and essential multiprotein complexes important for higher order chormatin structural organization in mitosis, gene regulation and DNA repair. In collaboration with Dr. Berns' laboratory, we are studying the mechanism and regulation of DNA damage recognition by SMC complexes. We found that near IR laser at the BLI is uniquely suited for detecting the recruitment of one of the SMC complexes to damage sites. We plan to study the recruitment kinetics and factor requirement using the system. The study will provides important insight into the role of chromatin regulators in DNA repair, which may serve as possible therapeutic targets for cancer treatment in the future.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 SMC复合体是一种保守的、重要的多蛋白复合体，在有丝分裂、基因调控和DNA修复中对高阶Chmatin结构组织起重要作用。与伯恩斯博士的实验室合作，我们正在研究SMC复合体识别DNA损伤的机制和调控。我们发现，BLI的近红外激光非常适合于探测SMC复合体之一对损伤部位的招募。我们计划使用该系统研究招聘动力学和因素需求。这项研究将为染色质调节因子在DNA修复中的作用提供重要的见解，这可能成为未来癌症治疗的可能治疗靶点。