ROLE OF SMC COMPLEXES IN DNA REPAIR

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 SMC复合物是保守的和必需的多蛋白复合物，对于有丝分裂、基因调控和DNA修复中的高级染色质结构组织非常重要。 与Berns博士的实验室合作，我们正在研究SMC复合物识别DNA损伤的机制和调控。 我们发现，近红外激光在BLI是唯一适合于检测招聘的SMC复合物之一，以损害网站。 我们计划使用该系统来研究募集动力学和因子需求。 这项研究将为染色质调节剂在DNA修复中的作用提供重要的见解，这可能成为未来癌症治疗的可能治疗靶点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. SMC complexes are conserved and essential multiprotein complexes important for higher order chormatin structural organization in mitosis, gene regulation and DNA repair. In collaboration with Dr. Berns' laboratory, we are studying the mechanism and regulation of DNA damage recognition by SMC complexes. We found that near IR laser at the BLI is uniquely suited for detecting the recruitment of one of the SMC complexes to damage sites. We plan to study the recruitment kinetics and factor requirement using the system. The study will provides important insight into the role of chromatin regulators in DNA repair, which may serve as possible therapeutic targets for cancer treatment in the future.