MULTI-PHOTON IMAGING OF ACTIN FILAMENT FORMATION AND MITOCHONDRIAL ENERGETICS

肌动蛋白丝形成和线粒体能量的多光子成像

• 批准号: 8362658
• 负责人: TATIANA B KRASIEVA
• 金额: $ 1.03万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362658
• 关键词: Biological Models; Biotechnology; Cancer Model; Cell Survival; Cells; Coupling; Differentiation and Growth; Dyes; Energy Metabolism; Evaluation; Extracellular Matrix; F-Actin; Flavin-Adenine Dinucleotide; Fluorescence; Fluorometry; Funding; Glioblastoma; Glioma; Grant; Human; Image; Lasers; Malignant Glioma; Malignant Neoplasms; Measurement; Metabolic; Methods; Microfilaments; Mitochondria; NADH; National Center for Research Resources; Neoplasm Metastasis; Nicotinamide adenine dinucleotide; Oxidation-Reduction; Principal Investigator; Property; Research; Research Infrastructure; Research Personnel; Resources; Signal Transduction; Source; United States National Institutes of Health; base; cost; imaging modality; in vivo; mitochondrial membrane; multi-photon; optical imaging; outcome forecast; ratiometric; response; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. PI will be developing multiphoton (MPM) optical imaging methods to systematically study human glioblastoma multiform spheroids embedded in various 3D ECMs. These experimental cancer model systems are used to mimic the in vivo gliomas. An MPM ratiometric redox fluorometry method based on intrinsic cellular fluorescence from reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavin adenine dinucleotide (FAD) to study mitochondrial energy metabolism will be developed by the PI. Coupling these finding with the evaluations of mitochondrial membrane using a dye and visualizing F-actin distribution in conjunction with mitochondrial signals will furnish a better understanding on how ECM alters the growth, differentiation and metabolic states of the malignant glioma cells. The imaging methods developed will help cancer investigators to study key cancer properties such as cell survival and metastasis and can allow accurate measurements of tumor response to treatments. The studies proposed will benefit current therapies and prognosis for glioblastoma multiform.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 PI 将开发多光子 (MPM) 光学成像方法，以系统地研究嵌入各种 3D ECM 中的人类胶质母细胞瘤多形球体。这些实验性癌症模型系统用于模拟体内神经胶质瘤。 PI 将开发一种基于还原烟酰胺腺嘌呤二核苷酸 (NADH) 和氧化黄素腺嘌呤二核苷酸 (FAD) 固有细胞荧光的 MPM 比率氧化还原荧光测定方法，用于研究线粒体能量代谢。 将这些发现与使用染料对线粒体膜的评估结合起来，并将 F-肌动蛋白分布与线粒体信号结合起来可视化，将有助于更好地理解 ECM 如何改变恶性胶质瘤细胞的生长、分化和代谢状态。 开发的成像方法将帮助癌症研究人员研究细胞存活和转移等关键癌症特性，并可以准确测量肿瘤对治疗的反应。拟议的研究将有利于多形性胶质母细胞瘤的当前治疗和预后。